Growth Inhibition of Thermotolerant Yeast, Kluyveromyces marxianus, in Hydrolysates from Cassava Pulp

In this study, we report the inhibition of Kluyveromyces marxianus TISTR5925 growth and ethanol fermentation in the presence of furan derivatives and weak acids (acetic acid and lactic acid) at high temperatures. Cassava pulp, obtained as the waste from starch processing, was collected from 14 starc...

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Published in:Applied biochemistry and biotechnology 2014-07, Vol.173 (5), p.1197-1208
Main Authors: Rugthaworn, Prapassorn, Murata, Yoshinori, Machida, Masashi, Apiwatanapiwat, Waraporn, Hirooka, Akiko, Thanapase, Warunee, Dangjarean, Hatairat, Ushiwaka, Satoru, Morimitsu, Kozo, Kosugi, Akihiko, Arai, Takamitsu, Vaithanomsat, Pilanee
Format: Article
Language:English
Subjects:
Acetic acid
Acetic Acid - pharmacology
Acids
Biochemistry
Biological and medical sciences
Biotechnology
cassava
Chemistry
Chemistry and Materials Science
Ethanol
Ethanol - metabolism
ethanol fermentation
ethanol production
Factories
Fermentation
Fermentation - drug effects
Fundamental and applied biological sciences. Psychology
Furans - pharmacology
growth retardation
heat tolerance
high performance liquid chromatography
High temperature
hydrolysates
Hydrolysis
Kluyveromyces - drug effects
Kluyveromyces - genetics
Kluyveromyces - growth & development
Kluyveromyces - metabolism
Kluyveromyces marxianus
lactic acid
Lactic Acid - pharmacology
Liquid chromatography
Manihot - chemistry
Manihot esculenta
Mutagenesis
mutants
Mutation
Plant growth
pulp
Saccharomyces cerevisiae
Starch
Temperature
tryptophan
Tryptophan - pharmacology
wastes
Yeast
Yeasts
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creator Rugthaworn, Prapassorn
Murata, Yoshinori
Machida, Masashi
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Thanapase, Warunee
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Ushiwaka, Satoru
Morimitsu, Kozo
Kosugi, Akihiko
Arai, Takamitsu
Vaithanomsat, Pilanee
description In this study, we report the inhibition of Kluyveromyces marxianus TISTR5925 growth and ethanol fermentation in the presence of furan derivatives and weak acids (acetic acid and lactic acid) at high temperatures. Cassava pulp, obtained as the waste from starch processing, was collected from 14 starch factories located in several provinces of Thailand. At a high temperature (42 °C), the cassava pulp hydrolysate from some starch factories strongly inhibited growth and ethanol production of both K. marxianus (strain TISTR5925) and Saccharomyces cerevisiae (strain K3). HPLC detected high levels of lactic acid and acetic acid in the hydrolysates, suggesting that these weak acids impaired the growth of K. marxianus at high temperature. We isolated Trp-requiring mutants that had reduced tolerance to acetic acid compared to the wild-type. This sensitivity to acetic acid was suppressed by supplementation of the medium with tryptophan.
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subjects Acetic acid
Acetic Acid - pharmacology
Acids
Biochemistry
Biological and medical sciences
Biotechnology
cassava
Chemistry
Chemistry and Materials Science
Ethanol
Ethanol - metabolism
ethanol fermentation
ethanol production
Factories
Fermentation
Fermentation - drug effects
Fundamental and applied biological sciences. Psychology
Furans - pharmacology
growth retardation
heat tolerance
high performance liquid chromatography
High temperature
hydrolysates
Hydrolysis
Kluyveromyces - drug effects
Kluyveromyces - genetics
Kluyveromyces - growth & development
Kluyveromyces - metabolism
Kluyveromyces marxianus
lactic acid
Lactic Acid - pharmacology
Liquid chromatography
Manihot - chemistry
Manihot esculenta
Mutagenesis
mutants
Mutation
Plant growth
pulp
Saccharomyces cerevisiae
Starch
Temperature
tryptophan
Tryptophan - pharmacology
wastes
Yeast
Yeasts
title Growth Inhibition of Thermotolerant Yeast, Kluyveromyces marxianus, in Hydrolysates from Cassava Pulp
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