Toxicokinetic study of pyrrole adducts and its potential application for biological monitoring of 2,5-hexanedione subacute exposure

• Published in: International archives of occupational and environmental health 2014-08, Vol.87 (6), p.655-662
• Main Authors: Yin, Hong-Yin, Guo, Ying, Song, Fu-Yong, Zeng, Tao, Xie, Ke-Qin
• Format: Article
• Language: English
• Subjects: Animals; Area Under Curve; Biological and medical sciences; Biomarkers; Biomarkers - blood; Biomarkers - urine; Biomonitoring; Chemical and industrial products toxicology. Toxic occupational diseases; Earth and Environmental Science; Environment; Environmental Health; Environmental Monitoring; Gas chromatography; Hexanes - metabolism; Hexanes - toxicity; Hexanones - metabolism; Hexanones - toxicity; Kinetics; Male; Medical sciences; Neurotoxins - metabolism; Neurotoxins - toxicity; Occupational diseases; Occupational medicine; Occupational Medicine/Industrial Medicine; Original Article; Public health. Hygiene-occupational medicine; Pyrroles - metabolism; Pyrroles - toxicity; Rats; Rats, Wistar; Rehabilitation; Solvents; Spectrophotometry; Toxicity; Toxicokinetics; Toxicology; Urine
• ISSN: 0340-0131; 1432-1246
• DOI: 10.1007/s00420-013-0907-4

Purpose The formation of pyrrole adducts might be responsible for peripheral nerve injury caused by n -hexane, but there is not an effective biomarker for monitoring occupational exposure of n -hexane. The current study was designed to investigate the changes of pyrrole adducts in serum and urine of rats exposed to 2,5-hexanedione (2,5-HD) and analyze the correlation between pyrrole adducts and 2,5-HD. Methods Two groups of male Wistar rats ( n  = 8) were administered a single dose of 200 and 400 mg/kg 2,5-HD (i.p.), and another two groups ( n  = 8) were given daily dose of 200 and 400 mg/kg 2,5-HD (i.p.) for 5 days. Pyrrole adducts and 2,5-HD in serum and urine were determined, at different time points after dosing, using Ehrlich’s reagent and gas chromatography, respectively. Results The levels of pyrrole adducts in serum accumulated in a time-dependant manner after repeated exposure to 2,5-HD, while pyrrole adducts in urine, and 2,5-HD in serum and urine were kept stable. The half-life times ( t 1/2 ) of 2,5-HD and pyrrole adducts in serum were 2.27 ± 0.28 and 25.3 ± 3.34 h, respectively. Furthermore, the levels of pyrrole adducts in urine were significantly correlated with the levels of 2,5-HD in serum ( r  = 0.736, P