Assembly and nuclear export of pre-ribosomal particles in budding yeast

The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 spe...

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Bibliographic Details
Published in:Chromosoma 2014-08, Vol.123 (4), p.327-344
Main Authors: Gerhardy, Stefan, Menet, Anna Maria, Peña, Cohue, Petkowski, Janusz Jurand, Panse, Vikram Govind
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Animal Genetics and Genomics
Biochemistry
Biomedical and Life Sciences
Cell Biology
cell nucleolus
Cell Nucleus - metabolism
Developmental Biology
enzymes
eukaryotic cells
Eukaryotic Microbiology
Human Genetics
Life Sciences
Models, Molecular
nucleoporins
physiological transport
Review Article
Ribosome Subunits - metabolism
ribosomes
Ribosomes - metabolism
Saccharomyces cerevisiae
Saccharomycetales - metabolism
yeasts
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Summary:The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that associate with pre-ribosomal particles at distinct stages to perform specific assembly steps. On their way through the nucleus, diverse energy-consuming enzymes are thought to release assembly factors from maturing pre-ribosomal particles after accomplishing their task(s). Subsequently, recruitment of export factors prepares pre-ribosomal particles for transport through nuclear pore complexes. Pre-ribosomes are exported into the cytoplasm in a functionally inactive state, where they undergo final maturation before initiating translation. Accumulating evidence indicates a tight coupling between nuclear export, cytoplasmic maturation, and final proofreading of the ribosome. In this review, we summarize our current understanding of nuclear export of pre-ribosomal subunits and cytoplasmic maturation steps that render pre-ribosomal subunits translation-competent.
ISSN:0009-5915
1432-0886
DOI:10.1007/s00412-014-0463-z