Spin equilibrium relaxation kinetics of cytochrome P450 LM2

The relaxation kinetics of the temperature-dependent spin equilibrium of cytochrome P450 LM2 has been resolved by means of a laser temperature-jump device. The Nd laser system in the Q-switched mode delivers 3 J/25 ns pulses which were Raman shifted to 1.89 μm by use of a high pressure H 2-gas cell....

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Bibliographic Details
Published in:FEBS letters 1982-12, Vol.150 (1), p.219-222
Main Authors: Ziegler, M., Blanck, J., Ruckpaul, K.
Format: Article
Language:English
Subjects:
Cytochrome P450
cytocochrome P-450
Laser
liver
microsomes
rabbits
Raman spectroscopy
Reaction mechanism
Relaxation kinetics
Spin equilibrium
spin transitions
Temperature-jump
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Summary:The relaxation kinetics of the temperature-dependent spin equilibrium of cytochrome P450 LM2 has been resolved by means of a laser temperature-jump device. The Nd laser system in the Q-switched mode delivers 3 J/25 ns pulses which were Raman shifted to 1.89 μm by use of a high pressure H 2-gas cell. The LM2 equilibrium relaxation proceeds in the ns-range, the rate constants of the on- and off-reactions amount to about 10 6 s −1 in correspondence with respective processes of other hemoproteins and protein-free model compounds. Benzphetamine increases the on-rate constant (low-spin → high-spin) about 5 fold, the off-process is nearly unchanged.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(82)81338-0