Assessment of the in Vitro Radiosensitivity of Human Peripheral Blood Lymphocytes

The proliferative capacity of sensitive lymphocyte progenitor cells from 31 clinically normal adults was evaluated following in vitro X irradiation at 0-400 R. Radiation effects were studied using both whole blood and density gradient-separated mononuclear cells in the lymphocyte stimulation test (L...

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Bibliographic Details
Published in:Radiation research 1982-03, Vol.89 (3), p.575-589
Main Authors: Knox, S. J., Shifrine, M., Rosenblatt, L. S.
Format: Article
Language:English
Subjects:
B lymphocytes
Blood
Cell Division - radiation effects
Cell Separation
Cell Survival - radiation effects
Colony-Forming Units Assay
Dose response relationship
Dose-Response Relationship, Radiation
Humans
In Vitro Techniques
Irradiation
Lymphocytes
Lymphocytes - drug effects
Lymphocytes - radiation effects
Methods
Mitogens - pharmacology
Mixed lymphocyte culture test
Probability
Radiation effects
Radiation Tolerance
T lymphocytes
Tuberculin
X-Rays
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Summary:The proliferative capacity of sensitive lymphocyte progenitor cells from 31 clinically normal adults was evaluated following in vitro X irradiation at 0-400 R. Radiation effects were studied using both whole blood and density gradient-separated mononuclear cells in the lymphocyte stimulation test (LST) and colony formation assay with six different mitogens/antigens. The sensitivities of the different methods are compared. Generally, assays using whole blood were capable of detecting radiation effects at lower dose levels than assays using gradient-separated cells. The colony formation assay was consistently more radiosensitive than the LST within mitogen/antigen groups. Dose-response curves were determined for the different test groups. Survival curves based upon the LST were characterized by a typical dose-response curve with a shoulder at low dose levels, followed by a single-exponential decrease. The number of colonies relative to dose generally followed a two-exponential curve that lacked a shoulder at lower doses, indicating the presence of at least two components with differing radiosensitivity. The biological significance of these findings is discussed. Normative values presented here may be useful for the determination of the relative radiosensitivity of lymphocytes from different population groups.
ISSN:0033-7587
1938-5404
DOI:10.2307/3575625