The effects of daily supplementation of Dendrobium huoshanense polysaccharide on ethanol-induced subacute liver injury in mice by proteomic analysis

Polysaccharides isolated from edible Dendrobium huoshanense have been shown to possess a hepatoprotection function for selenium- and carbon tetrachloride-induced liver injury. In this study, we investigated the preventive effects of daily supplementation with an homogeneous polysaccharide (DHP) puri...

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Bibliographic Details
Published in:Food & function 2014-09, Vol.5 (9), p.2020-2035
Main Authors: Wang, Xiao-Yu, Luo, Jian-Ping, Chen, Rui, Zha, Xue-Qiang, Wang, He
Format: Article
Language:English
Subjects:
Animals
Dendrobium - chemistry
Dietary Supplements - analysis
Ethanol - adverse effects
Humans
Liver - chemistry
Liver - metabolism
Liver Diseases, Alcoholic - drug therapy
Liver Diseases, Alcoholic - genetics
Liver Diseases, Alcoholic - metabolism
Male
Mice
Plant Extracts - administration & dosage
Polysaccharides - administration & dosage
Proteomics
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Summary:Polysaccharides isolated from edible Dendrobium huoshanense have been shown to possess a hepatoprotection function for selenium- and carbon tetrachloride-induced liver injury. In this study, we investigated the preventive effects of daily supplementation with an homogeneous polysaccharide (DHP) purified from D. huoshanense on ethanol-induced subacute liver injury in mice and its potential mechanisms in liver protection by a proteomic approach. DHP was found to effectively depress the increased ratio of liver weight to body weight, reduce the elevated levels of serum aspartate aminotransferase, total cholesterol, total bilirubin and low density lipoprotein, and alleviate hepatic steatosis in mice with ethanol-induced subacute liver injury. Hepatic proteomics analysis performed by two-dimensional difference gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) revealed that cystathionine beta-synthase (Cbs) and D-lactate dehydrogenase (Ldhd) were two key proteins regulated by daily DHP intervention, which may assist in correcting the abnormal hepatic methionine metabolism pathway and decreasing the level of hepatic methylglyoxal generated from disordered metabolic pathways caused by ethanol. Our data suggest that DHP can protect liver function from alcoholic injury with complicated molecular mechanisms involving regulation of Cbs and Ldhd.
ISSN:2042-6496
2042-650X
DOI:10.1039/c3fo60629e