The Usage Of Phage Mini-Antibodies As A Means Of Detecting Ferritin Concentration In Animal Blood Serum

Mini-antibodies that have specific ferritin response have been produced for the first time using sheep's phage libraries (Griffin.1, Medical Research Council, Cambridge, UK). Produced phage antibodies were used for the first time for the development of diagnostic test kits for ferritin detectio...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2015-01, Vol.36 (1), p.100-110
Main Authors: Staroverov, Sergey A., Volkov, Alexei A., Fomin, Alexander S., Laskavuy, Vladislav N., Mezhennyy, Pavel V., Kozlov, Sergey V., Larionov, Sergey V., Fedorov, Michael V., Dykman, Lev A., Guliy, Olga I.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - immunology
Antibody Specificity - immunology
Cattle
colloidal gold
ferritin
Ferritins - blood
Ferritins - immunology
Ferritins - isolation & purification
Immunoblotting
immunodot
Liver - chemistry
Liver - immunology
nanoshells
phage libraries
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Summary:Mini-antibodies that have specific ferritin response have been produced for the first time using sheep's phage libraries (Griffin.1, Medical Research Council, Cambridge, UK). Produced phage antibodies were used for the first time for the development of diagnostic test kits for ferritin detection in the blood of cattle. The immunodot assay with secondary biospecific labeling is suggested as means of ferritin detection in cow blood serum (antiferritin phage antibodies and rabbit antiphage antibodies conjugated with different labels). Сolloidal gold, gold nanoshells, and horse reddish peroxidase used as labels have shown a similar response while detecting concentration of ferritin (0.2 mg/mL). It is shown that the method of solid-phase immunoassay with a visual view of the results allows determination of the minimum concentration of ferritin in the blood of cows at 0.225 g/mL.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2014.899257