Study of simian adenovirus genome. II. Identification of specific fragment of viral DNA with transforming activity

Simian adenovirus DNA was fragmented using restriction enzymes EcoRI, BamHI, XbaI, and SalI. The mixtures of restriction fragment formed as a result were used to transform a primary culture of baby rat kidney cells. The DNA fragments obtained after treatment of the viral DNA with enzymes BamHI, XbaI...

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Bibliographic Details
Published in:Biotechnology letters 1982-01, Vol.4 (7), p.255-259
Main Authors: Gibadulin, R A, Dijkema, R, Denisova, T S, van Ormondt, H
Format: Article
Language:English
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Summary:Simian adenovirus DNA was fragmented using restriction enzymes EcoRI, BamHI, XbaI, and SalI. The mixtures of restriction fragment formed as a result were used to transform a primary culture of baby rat kidney cells. The DNA fragments obtained after treatment of the viral DNA with enzymes BamHI, XbaI, and SalI were capable of transforming cultured cells as well as native viral DNA. The transforming activity of individual specific DNA fragments obtained after cleavage of the viral DNA by restriction enzymes BamHI and SalI was studied. It was established that specific fragments of the simian adenovirus DNAs studied (BamHI-B and SalI-B), located at the left end of a physical map of the viral geonome, were previously oriented on the basis of the content of GC pairs in the DNA molecule. It was shown that cells of the transformed lines are capable of growing in the form of colonies in a semiliquid medium containing 0.33% agarose.
ISSN:0141-5492