RNA-dependent RNA Polymerase Activity in Murine Coronavirus-infected Cells

Institut für Virologie und Immunbiologie, Universität Würzburg, Versbacher Strasse 7, D8700 Würzburg, Federal Republic of Germany The multiplication of murine coronavirus strains A59 or JHM in Sac(-) cells was unaffected by the presence of -amanitin at concentrations which inhibited the host cell DN...

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Bibliographic Details
Published in:Journal of general virology 1983-01, Vol.64 (1), p.103-111
Main Authors: Mahy, Brian W. J, Siddell, Stuart, Wege, Helmut, Ter Meulen, Volker
Format: Article
Language:English
Subjects:
Amanitins - pharmacology
Animals
Anisomycin - pharmacology
Cell Line
Dactinomycin - pharmacology
Hydrogen-Ion Concentration
Kinetics
Magnesium - pharmacology
Mice
Murine hepatitis virus - enzymology
Murine hepatitis virus - growth & development
RNA Nucleotidyltransferases - metabolism
RNA Replicase - metabolism
RNA, Viral - biosynthesis
Temperature
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Summary:Institut für Virologie und Immunbiologie, Universität Würzburg, Versbacher Strasse 7, D8700 Würzburg, Federal Republic of Germany The multiplication of murine coronavirus strains A59 or JHM in Sac(-) cells was unaffected by the presence of -amanitin at concentrations which inhibited the host cell DNA-dependent RNA polymerase activity. In cells infected with the A59 virus strain, actinomycin D-resistant RNA synthesis could readily be detected by pulse-labelling with [ 3 H]uridine; this virus-specific RNA synthesis was not induced in the presence of the protein synthesis inhibitor anisomycin. A new RNA-dependent RNA polymerase activity was detected in the large particle fraction of A59 virus-infected cells. Optimal conditions for enzyme activity in vitro were established. Maximum activity occurred 5 h after infection, coincident with the peak of virus-specific RNA synthesis detected by pulse-labelling in vivo . Keywords: RNA-dependent RNA polymerase, -amanitin, coronavirus replication, Sac(-) cells Present address: Division of Virology, Department of Pathology, University of Cambridge, Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, U.K. Received 24 May 1982; accepted 29 July 1982.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-64-1-103