Sensitive and rapid amino acid analysis of peptide hydrolysates by high-performance liquid chromatography of o-phthaldialdehyde derivatives

In this paper a solvent system optimized for the Nucleosil 5 C sub(18) column is described. This system separates OPT derivatives of all amino acids common to peptide hydrolysates with good resolution. An evaluation of chromatographic conditions and quantitative properties is presented, resulting in...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1982-01, Vol.237 (2), p.339-343
Main Authors: Burbach, J.Peter H., Prins, Arie, Lebouille, Jos L.M., Verhoef, J., Witter, Albert
Format: Article
Language:English
Subjects:
amino acid analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper a solvent system optimized for the Nucleosil 5 C sub(18) column is described. This system separates OPT derivatives of all amino acids common to peptide hydrolysates with good resolution. An evaluation of chromatographic conditions and quantitative properties is presented, resulting in a reproducible and sensitive method for the amino acid analysis of small amounts of biologically active peptides.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)83245-8