Local and systemic immune responses induced by a recombinant chimeric protein containing Mycoplasma hyopneumoniae antigens fused to the B subunit of Escherichia coli heat-labile enterotoxin LTB

•A chimera with three antigens of M. hyopneumoniae and adjuvant LTB was constructed.•Antigenic and immunogenic properties were evaluated in mice and pigs.•Antibodies against each subunit recognized the chimeric protein.•The adjuvant rLTB enhanced the immune response in fused and co-administered anti...

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Bibliographic Details
Published in:Veterinary microbiology 2014-09, Vol.173 (1-2), p.166-171
Main Authors: Marchioro, Silvana Beutinger, Fisch, Andressa, Gomes, Charles K., Jorge, Sérgio, Galli, Vanessa, Haesebrouck, Freddy, Maes, Dominiek, Dellagostin, Odir, Conceição, Fabricio R.
Format: Article
Language:English
Subjects:
Adjuvant
Adjuvants, Immunologic - chemistry
Administration, Intranasal
Animals
Antibodies, Bacterial - blood
Bacterial enterotoxins
Enterotoxins - chemistry
Enterotoxins - immunology
Escherichia coli - chemistry
Female
Fused protein
Immunoglobulin A - blood
Immunoglobulin G - blood
Injections, Intramuscular
Mice
Mice, Inbred BALB C
Mycoplasma hyopneumoniae - chemistry
Mycoplasma hyopneumoniae - immunology
Pneumonia of Swine, Mycoplasmal - immunology
Pneumonia of Swine, Mycoplasmal - microbiology
Pneumonia of Swine, Mycoplasmal - prevention & control
Recombinant Fusion Proteins - administration & dosage
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Swine
Swine enzootic pneumonia
Vaccination
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Summary:•A chimera with three antigens of M. hyopneumoniae and adjuvant LTB was constructed.•Antigenic and immunogenic properties were evaluated in mice and pigs.•Antibodies against each subunit recognized the chimeric protein.•The adjuvant rLTB enhanced the immune response in fused and co-administered antigens. A multi-antigen chimera composed of three antigens of Mycoplasma hyopneumoniae (R1, P42, and NrdF) and the mucosal adjuvant Escherichia coli heat-labile enterotoxin B subunit (LTB) was constructed, and its antigenic and immunogenic properties were evaluated in mice and pigs. In addition, we compared the effect of the fusion and co-administration of these proteins in mice. Antibodies against each subunit recognized the chimeric protein. Intranasal and intramuscular immunization of mice with the chimeric protein significantly increased IgG and IgA levels in the serum and tracheobronchial lavages, respectively, against some of the antigens present in the chimeric. Swine immunized with the chimeric protein developed an immune response against all M. hyopneumoniae antigens present in the fusion with a statistically significant difference (P
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2014.07.009