Localization of axonally transported super(125)I-wheat germ agglutinin beneath the plasma membrane of chick retinal ganglion cells

The distribution of super(125)I-wheat germ agglutinin (WGA) transported by axons of chick retinal ganglion cells to layer d of the optic tectum was studied by electron microscopic autoradiography. The authors found that 52% of the radioactivity was loacted in axons and axon terminals in the contrala...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1983-01, Vol.96 (2), p.373-381
Main Authors: LaVail, J H, Sugino, I K, McDonald, D M
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The distribution of super(125)I-wheat germ agglutinin (WGA) transported by axons of chick retinal ganglion cells to layer d of the optic tectum was studied by electron microscopic autoradiography. The authors found that 52% of the radioactivity was loacted in axons and axon terminals in the contralateral optic tectum 22 h after intravitreal injection of affinity-purified super(125)I-WGA. Axon comprised 43% of the volume of layer d. Dendrites, glial cells, and neuron cell bodies contained 20%, 17%, and 3% of the label, whereas these structures comprised 24%, 21%, and 2% of the tissue volume, respectively.
ISSN:0021-9525