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Increased Cytosolic pH in Multidrug-Resistant Human Lung Tumor Cells: Effect of Verapamil

In a set of four increasingly multidrug-resistant variants of SW-1573 human lung tumor cells, the pHi (i.e., steady-state cytosolic pH) increased up to 0.44 U as a function of the level of doxorubicin resistance. The elevated pHi in the most resistant (2, 000-fold) variant dropped to the control lev...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1989-05, Vol.81 (9), p.706-709
Main Authors: Keizer, Hiskias G., Joenje, Hans
Format: Article
Language:English
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Summary:In a set of four increasingly multidrug-resistant variants of SW-1573 human lung tumor cells, the pHi (i.e., steady-state cytosolic pH) increased up to 0.44 U as a function of the level of doxorubicin resistance. The elevated pHi in the most resistant (2, 000-fold) variant dropped to the control level upon addition of verapamil, a known inhibitor of P-glycoprotein activity. These data suggest that, in the absence of xenobiotic substrates, P-glycoprotein activity can affect cellular pHi. This finding may be important for the elucidation of the physiological function of this protein. [J Natl Cancer Inst 81: 706–709, 1989]
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/81.9.706