Synergy between penicillin and gentamicin against enterococci

The role of active uptake in aminoglycoside activity against penicillin-treated enterococci was studied by viable counts and ATP determinations. Penicillin and gentamicin gave synergistic bactericidal and post-antibiotic effects (PAEs) which were partially reduced by sodium aride, an electron transp...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 1990-04, Vol.25 (4), p.551-560
Main Authors: Winstanley, T. G., Hastings, J. G. M.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Azides - pharmacology
Drug Synergism
Edetic Acid - pharmacology
Gentamicins - pharmacology
Microbial Sensitivity Tests
Penicillins - pharmacology
Sodium Azide
Streptococcus
Streptococcus - drug effects
Streptococcus - metabolism
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Summary:The role of active uptake in aminoglycoside activity against penicillin-treated enterococci was studied by viable counts and ATP determinations. Penicillin and gentamicin gave synergistic bactericidal and post-antibiotic effects (PAEs) which were partially reduced by sodium aride, an electron transport inhibitor, and totally blocked in the presence of both sodium azide and EDTA, which chelates divalent cations. EDTA and gentamicin showed marked synergy in both ‘killing curve’ and PAE experiments. This synergy was completely inhibited by sodium azide. The data indicate that the activity of gentamicin against enterococci that have been damaged by penicillin or EDTA is energy-dependent. This is consistent with present theories of gentamicin uptake via transportation driven by a protonmotive force.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/25.4.551