Detection of 1q polysomy in interphase nuclei of human solid tumors with a biotinylated probe

A biotinylated probe (L23-21) specific for the 1q12 band of human karyotype was used to detect the 1q segment in interphase nuclei of breast and colon carcinomas. This probe was selected because trisomy or polysomy 1q is the most frequent chromosomal change observed in solid tumors. This method enab...

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Bibliographic Details
Published in:Human genetics 1989-03, Vol.81 (4), p.311-314
Main Authors: Viegas-Péquignot, E, Jeanpierre, M, Dutrillaux, A M, Gerbault-Seureau, M, Muleris, M, Dutrillaux, B
Format: Article
Language:English
Subjects:
Biotin
Breast Neoplasms - genetics
Carcinoma, Intraductal, Noninfiltrating - genetics
Cell Nucleus - ultrastructure
Cell Separation
Chromosomes, Human, Pair 1
Colonic Neoplasms - genetics
DNA Probes
Flow Cytometry
Humans
Interphase
Karyotyping
Trisomy
Tumor Cells, Cultured
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Summary:A biotinylated probe (L23-21) specific for the 1q12 band of human karyotype was used to detect the 1q segment in interphase nuclei of breast and colon carcinomas. This probe was selected because trisomy or polysomy 1q is the most frequent chromosomal change observed in solid tumors. This method enables cancerous cells, including near-diploid ones carrying an unbalanced rearrangement of 1q, to be easily identified.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00283681