Cerebral metabolism of acetate and glucose studied by super(13)C-n.m.r. spectroscopy. A technique for investigating metabolic compartmentation in the brain

The time courses of incorporation of super(13)C from super(13)C-labelled glucose or acetate into cerebral amino acids (glutamate, glutamine and 4-aminobutyrate) and lactate were monitored by using super(13)C-n.m.r. spectroscopy. When (1- super(13)C)glucose was used as precursor the C-2 of 4-aminobut...

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Bibliographic Details
Published in:Biochemical journal 1990-01, Vol.266 (1), p.133-139
Main Authors: Badar-Goffer, R S, Bachelard, H S, Morris, P G
Format: Article
Language:English
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Summary:The time courses of incorporation of super(13)C from super(13)C-labelled glucose or acetate into cerebral amino acids (glutamate, glutamine and 4-aminobutyrate) and lactate were monitored by using super(13)C-n.m.r. spectroscopy. When (1- super(13)C)glucose was used as precursor the C-2 of 4-aminobutyrate was more highly labelled than the analogous C-4 of glutamate, whereas no label was observed in glutamine. A similar pattern was observed with (2- super(13)C)glucose: the C-1 of 4-aminobutyrate was more highly labelled than the analogous C-5 of glutamate. The results are consistent with earlier patterns reported from the use of super(14)C-labelled precursors that led to the concept of compartmentation of neuronal and glial metabolism and now provide the possibility of distinguishing differential effects of metabolic perturbations on the two pools simultaneously.
ISSN:0264-6021