Mutation of glycine 49 to valine in the alpha subunit of G sub(s) results in the constitutive elevation of cyclic AMP synthesis

The G-protein G sub(s) couples hormone-activated receptors with adenylyl cyclase and stimulates increased cyclic AMP synthesis. Transient expression in COS-1 cells of cDNAs coding for the G sub(s) alpha -subunit ( alpha sub(s)) or alpha sub(s) cDNAs having simple amino acid mutations Gly49 arrow rig...

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Bibliographic Details
Published in:Biochemistry (Easton) 1989-01, Vol.28 (11), p.4547-4551
Main Authors: Woon, Chee Wai, Heasley, L, Osawa, S, Johnson, G L
Format: Article
Language:English
Subjects:
glycine
valine
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Summary:The G-protein G sub(s) couples hormone-activated receptors with adenylyl cyclase and stimulates increased cyclic AMP synthesis. Transient expression in COS-1 cells of cDNAs coding for the G sub(s) alpha -subunit ( alpha sub(s)) or alpha sub(s) cDNAs having simple amino acid mutations Gly49 arrow right Val or Gly225 arrow right Thr elevated cyclic AMP levels, resulting in the activation of cyclic AMP dependent protein kinase.
ISSN:0006-2960