Initial analysis of 750 MHz NMR spectra of selectively super(15)N-G,U labelled E. coli 5S rRNA

The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to E. coli 5S rRNA (120 nucleo...

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Bibliographic Details
Published in:FEBS letters 1996-01, Vol.385 (1-2), p.114-118
Main Authors: Gruene, M, Goerlach, M, Soskic, V, Klussmann, S, Bald, R, Fuerste, JP, Erdmann, V A, Brown, L R
Format: Article
Language:English
Subjects:
Escherichia coli
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Summary:The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to E. coli 5S rRNA (120 nucleotides) selectively labelled with super(15)N in guanine and uridine allows observation of base pairing patterns for a larger RNA molecule. We also present evidence that the fold of the E-domain of the 5S rRNA (nt 79-97) as a contiguous part of the 5S rRNA and as an isolated molecule is virtually the same.
ISSN:0014-5793