The Effect of PnTx2-6 Protein From Phoneutria nigriventer Spider Toxin on Improvement of Erectile Dysfunction in a Rat Model of Cavernous Nerve Injury

Objective To explore the therapeutic potential of PnTx2-6 injected 3 times a week for 4 weeks into the intracavernosal tissue in a rat model of bilateral cavernous nerve crush injury (BCNI). Methods Eight-week-old male Sprague-Dawley rats were randomly divided into the following 6 groups (n = 5 per...

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Published in:Urology (Ridgewood, N.J.) N.J.), 2014-09, Vol.84 (3), p.730.e9-730.e17
Main Authors: Jung, Ae Ryang, Choi, Yong Sun, Piao, Shuyu, Park, Yong Hyun, Shrestha, Kshitiz Raj, Jeon, Seung Hwan, Hong, Sung-Hoo, Kim, Sae Woong, Hwang, Tae-Kon, Kim, Ki Hean, Lee, Ji Youl
Format: Article
Language:English
Subjects:
Animals
Baculoviridae - metabolism
Cyclic GMP - metabolism
Disease Models, Animal
Endothelium - pathology
Erectile Dysfunction - drug therapy
Insecta
Male
Nerve Crush
Neuropeptides - administration & dosage
Neuropeptides - chemistry
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type I - metabolism
Penile Erection - drug effects
Penis - drug effects
Rats
Rats, Sprague-Dawley
Spider Venoms - chemistry
Spiders
Urology
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Summary:Objective To explore the therapeutic potential of PnTx2-6 injected 3 times a week for 4 weeks into the intracavernosal tissue in a rat model of bilateral cavernous nerve crush injury (BCNI). Methods Eight-week-old male Sprague-Dawley rats were randomly divided into the following 6 groups (n = 5 per group): age-matched control (normal group), BCNI (injury group), post-BCNI phosphate-buffered saline injection (PBS group), post-BCNI Sf9 cell-lysate injection (N/C group), post-BCNI injection of cell lysate from S9 cells infected with wild-type recombinant baculovirus (W/T group), and post-BCNI injection of cell lysate from S9 cells infected with recombinant baculovirus containing PnTx2-6 (PnTx2-6 group). Injections were delivered 3 times a week for 4 weeks. After 4 weeks, intracavernosal pressure-to-mean arterial pressure ratio, smooth muscle and collagen content via the Masson trichrome staining, levels of neural nitric oxide synthase, phosphoendothelial nitric oxide synthase, and cyclic guanosine monophosphate were all measured. Results The PnTx2-6 group showed significantly higher intracavernosal pressure-to-mean arterial pressure ratio ( P  
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2014.05.030