Immobilizing Gold Nanoparticles in Mesoporous Silica Covered Reduced Graphene Oxide: A Hybrid Material for Cancer Cell Detection through Hydrogen Peroxide Sensing

A new kind of two-dimensional (2-D) hybrid material (RGO-PMS@AuNPs), fabricated by the immobilization of ultrasmall gold nanoparticles (AuNPs, ∼3 nm) onto sandwich-like periodic mesopourous silica (PMS) coated reduced graphene oxide (RGO), was employed for both electrocatalytic application and cance...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2014-08, Vol.6 (16), p.13648-13656
Main Authors: Maji, Swarup Kumar, Sreejith, Sivaramapanicker, Mandal, Amal Kumar, Ma, Xing, Zhao, Yanli
Format: Article
Language:English
Subjects:
Electrochemical Techniques
Electrodes
Glucose - analysis
Gold - chemistry
Graphite - chemistry
HEK293 Cells
HeLa Cells
Hep G2 Cells
Humans
Hydrogen Peroxide - urine
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Neoplasms - diagnosis
Oxides - chemistry
Particle Size
Photoelectron Spectroscopy
Porosity
Reproducibility of Results
Silicon Dioxide - chemistry
Spectrum Analysis, Raman
X-Ray Diffraction
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Summary:A new kind of two-dimensional (2-D) hybrid material (RGO-PMS@AuNPs), fabricated by the immobilization of ultrasmall gold nanoparticles (AuNPs, ∼3 nm) onto sandwich-like periodic mesopourous silica (PMS) coated reduced graphene oxide (RGO), was employed for both electrocatalytic application and cancer cell detection. The hybrid-based electrode sensor showed attractive electrochemical performance for sensitive and selective nonenzymatic detection of hydrogen peroxide (H2O2) in 0.1 M phosphate buffered saline, with wide linear detection range (0.5 μM to 50 mM), low detection limit (60 nM), and good sensitivity (39.2 μA mM–1 cm–2), and without any interference by common interfering agents. In addition, the sensor exhibited a high capability for glucose sensing and H2O2 detection in human urine. More interestingly, the hybrid was found to be nontoxic, and the electrode sensor could sensitively detect a trace amount of H2O2 in a nanomolar level released from living tumor cells (HeLa and HepG2). Because the hybrid presents significant properties for the detection of bioactive species and certain cancerous cells by the synergistic effect from RGO, PMS, and AuNPs, it could be able to serve as a versatile platform for biosensing, bioanalysis, and biomedical applications.
ISSN:1944-8244
1944-8252
DOI:10.1021/am503110s