A modified acidic approach for DNA extraction from plant species containing high levels of secondary metabolites

Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of sec...

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Bibliographic Details
Published in:Genetics and molecular research 2014-08, Vol.13 (3), p.6497-6502
Main Authors: Cavallari, M M, Siqueira, M V B M, Val, T M, Pavanelli, J C, Monteiro, M, Grando, C, Pinheiro, J B, Zucchi, M I, Gimenes, M A
Format: Article
Language:English
Subjects:
Anacardium - chemistry
Anacardium - genetics
Buffers
Casearia - chemistry
Casearia - genetics
DNA, Plant - genetics
DNA, Plant - isolation & purification
Electrophoresis, Agar Gel
Hydrogen-Ion Concentration
Lippia - chemistry
Lippia - genetics
Liquid Phase Microextraction - methods
Microsatellite Repeats
Plant Leaves - chemistry
Plant Leaves - genetics
Polymerase Chain Reaction - standards
Polyphenols
Potassium Acetate
Sulfites
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Summary:Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of secondary metabolites. This protocol does not require the use of volatile toxic reagents such as mercaptoethanol, chloroform, or phenol and allows the extraction of high-quality DNA from wild and cultivated tropical species.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.August.25.13