Anti-angiogenic effect of auranofin on HUVECs in vitro and zebrafish in vivo

Angiogenesis plays an essential role in many physiological and pathological processes. Auranofin (Ridaura®), an important gold(I) complex, is used to treat rheumatoid arthritis. However, the effect of auranofin on blood vessel formation is still unclear. In this study, we investigated the anti-angio...

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Bibliographic Details
Published in:European journal of pharmacology 2014-10, Vol.740, p.240-247
Main Authors: He, Ming-Fang, Gao, Xiao-Ping, Li, Shu-Chun, He, Zhi-Heng, Chen, Ning, Wang, Yu-Bin, She, Jin-Xiong
Format: Article
Language:English
Subjects:
Angiogenesis inhibitor
Angiogenesis Inhibitors - pharmacology
Animals
Auranofin
Auranofin - pharmacology
Blood Vessels - drug effects
Blood Vessels - embryology
Blood Vessels - physiology
Cell Line, Tumor
Cell Movement - drug effects
Cell Proliferation - drug effects
Cells, Cultured
Embryo, Nonmammalian
Human Umbilical Vein Endothelial Cells - drug effects
Human Umbilical Vein Endothelial Cells - physiology
Humans
HUVEC
Thioredoxin-Disulfide Reductase - genetics
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor Receptor-1 - genetics
Vascular Endothelial Growth Factor Receptor-2 - genetics
Vascular Endothelial Growth Factor Receptor-2 - metabolism
Zebrafish
Zebrafish Proteins - genetics
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Summary:Angiogenesis plays an essential role in many physiological and pathological processes. Auranofin (Ridaura®), an important gold(I) complex, is used to treat rheumatoid arthritis. However, the effect of auranofin on blood vessel formation is still unclear. In this study, we investigated the anti-angiogenic activity of auranofin on human umbilical vein endothelial cells (HUVEC) in vitro and zebrafish in vivo. Our results showed that auranofin could inhibit the proliferation, migration and tube formation of HUVECs and disrupted the formation of intersegmental vessels and the subintestinal vessels of zebrafish embryos. Auranofin inhibited the phosphorylation of vascular endothelial growth factor 2 (p-VEGFR2) on HUVECs and suppressed the vascular endothelial growth factor (VEGF) signaling pathway (vegfa, flt-1, kdr) but not thioredoxin reductase (TrxR) on zebrafish. Our study suggested that auranofin might serve as a potential anti-angiogenic compound candidate.
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2014.07.034