Ratios between the abundance of messenger RNA and the corresponding protein of two growth-related genes, c-myc and vimentin, in leukemic blast cells

The abundance of the mRNAs of two growth-related genes, vimentin and c-myc , and that of the corresponding proteins have been studied in unstimulated and phytohemagglutinin-stimulated lymphocytes as well as in 18 populations of leukemic blast cells. The quantitative assay was carried out by densitom...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1990-04, Vol.50 (7), p.1988-1991
Main Authors: FERRARI, S, TAGLIAFICO, E, D'INCA, M, CECCHERELLI, G, MANFREDINI, R, SELLERI, L, DONELLI, A, SACCHI, S, TORELLI, G, TORELLI, U
Format: Article
Language:English
Subjects:
Biological and medical sciences
Hematologic and hematopoietic diseases
leukemia
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
vimentin
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Summary:The abundance of the mRNAs of two growth-related genes, vimentin and c-myc , and that of the corresponding proteins have been studied in unstimulated and phytohemagglutinin-stimulated lymphocytes as well as in 18 populations of leukemic blast cells. The quantitative assay was carried out by densitometric scanning of Northern and Western blots. In normal lymphocytes the mRNA and the protein of both genes were almost undetectable. The phytohemagglutinin stimulation led to a sharp increase of the mRNA and the proteins of vimentin and c-myc . The increase was followed by a progressive fall of the gene products.
ISSN:0008-5472
1538-7445