Laccase activities of Penicillium chrysogenum in relation to lignin degradation

An extracellular laccase capable of oxidizing ABTS (the diammonium salt of 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) was detected in ligninolytic cultures of Penicillium chrysogenum. By contrast, no lignin peroxidase, manganese-dependent peroxidase or aryl-alcohol oxidase was detect...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 1996, Vol.45 (3), p.399-403
Main Authors: Rodriguez, A, Falcon, M.A, Carnicero, A, Perestelo, F, Fuente, G. de la, Trojanowski, J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Mission oriented research
Penicillium chrysogenum
Physiology and metabolism
soil biology
soil science
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An extracellular laccase capable of oxidizing ABTS (the diammonium salt of 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) was detected in ligninolytic cultures of Penicillium chrysogenum. By contrast, no lignin peroxidase, manganese-dependent peroxidase or aryl-alcohol oxidase was detected at any time during culturing. Both ABTS laccase activity and mineralization of dehydrogenative polymerizate of coniferyl alcohol were regulated by the C/N ratio in the medium and partially inhibited in the presence of thioglycolic acid, suggesting that both events are associated. In the presence of several known laccase inducers neither ABTS laccase activity nor mineralization rates were enhanced. However, a new laccase was detected in P. chrysogenum, able to oxidize 2,6-dimethoxyphenol but not involved in lignin mineralization. Studies with the known ligninolytic basidiomycete Trametes villosa suggest that lignin degradation by this fungus also involves the action of laccase.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530050702