Production and regeneration of protoplasts of Pisolithus tinctorius

The production of protoplasts of the ectomycorrhizal fungus Pisolithus tinctorius , isolate Pt 571, was improved using young mycelium treated with Novozym-234 dissolved in 0.5 M mannitol in the proportion 20:1:0.15 (mg mycelium: mg enzyme:ml mannitol) and incubated at 25 degree C for 4 hours. Platin...

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Bibliographic Details
Published in:Biotechnology techniques 1990-01, Vol.4 (3), p.215-220
Main Authors: Anunciacao, C.E, Guimaraes, W.V, Araujo, E.F, Muchovej, R.M.C, Souza, D.R.N. e
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cell walls
ectomycorrhizae
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
lysis
Methods. Procedures. Technologies
mycelium
Pisolithus tinctorius
Plant cells and fungal cells
Protoplast preparation, cell fusion and regeneration
protoplasts
regeneration
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Summary:The production of protoplasts of the ectomycorrhizal fungus Pisolithus tinctorius , isolate Pt 571, was improved using young mycelium treated with Novozym-234 dissolved in 0.5 M mannitol in the proportion 20:1:0.15 (mg mycelium: mg enzyme:ml mannitol) and incubated at 25 degree C for 4 hours. Plating the protoplasts on the surface of solid medium containing 0.5 M mannitol increased the regeneration rate.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00222509