Preparation and characterization of catechin-grafted chitosan with antioxidant and antidiabetic potential

In the present study, the preparation, characterization, antioxidant and antidiabetic activities of catechin-grafted chitosan (catechin-g-chitosan) were investigated. The graft of catechin onto chitosan was achieved by redox system and confirmed using various instrumental methods. Proton nuclear mag...

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Bibliographic Details
Published in:International journal of biological macromolecules 2014-09, Vol.70, p.150-155
Main Authors: Zhu, Weili, Zhang, Zhanjun
Format: Article
Language:English
Subjects:
alpha-Amylases - antagonists & inhibitors
alpha-Glucosidases
Antidiabetic
Antioxidant
Antioxidants - chemistry
Antioxidants - pharmacology
Catechin
Catechin - chemistry
Chitosan
Chitosan - chemistry
Conjugate
Free Radical Scavengers - chemistry
Free Radical Scavengers - pharmacology
Glycoside Hydrolase Inhibitors - chemistry
Glycoside Hydrolase Inhibitors - pharmacology
Hypoglycemic Agents - chemistry
Hypoglycemic Agents - pharmacology
Nuclear Magnetic Resonance, Biomolecular
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Summary:In the present study, the preparation, characterization, antioxidant and antidiabetic activities of catechin-grafted chitosan (catechin-g-chitosan) were investigated. The graft of catechin onto chitosan was achieved by redox system and confirmed using various instrumental methods. Proton nuclear magnetic resonance spectroscopy indicates that catechin has been successfully grafted onto chitosan. The morphology observation shows that chitosan changes to a softened nature with porous surface after grafting. Catechin-g-chitosan also exhibits reduced thermal stability and enhanced crystallinity compared to chitosan. Moreover, catechin-g-chitosan shows 0.51 of reducing power, 46.81% of hydroxyl radical-scavenging activity and 67.08% of DPPH radical-scavenging activity at 1mg/ml, which are much higher than that of chitosan. The antidiabetic activity in vitro assays shows that the α-glucosidase inhibitory effect decreases in the order of catechin-g-chitosan>catechin>acarbose>chitosan, and the α-amylase inhibitory effect decreases in the order of acarbose>catechin-g-chitosan>catechin>chitosan. The improved antioxidant and antidiabetic activities of catechin-g-chitosan are attributed to the phenolic groups in the catechin residues.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.06.047