Simultaneous separation and determination of four phenylethanoid glycosides in rat plasma sample after oral administration of Cistanche salsa extract by microemulsion liquid chromatography

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2014-03, Vol.951-952, p.24-31
• Main Authors: Zhou, Jun, Zhang, Qiong, Sun, Jiang Bing, Wang, Feng Qiao, Zeng, Ping
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Calibration; Chromatography, High Pressure Liquid; Chromatography, Liquid - methods; Cistanche - chemistry; Cistanche salsa; Drug Stability; Flow rate; Glycosides; Glycosides - blood; Glycosides - chemistry; Glycosides - isolation & purification; Hydrogen-Ion Concentration; Liquid chromatography; Male; Microemulsion liquid chromatography; Microemulsions; Optimization; Phenylethanoid glycosides; Phosphoric acid; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Rat plasma; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Separation
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2013.11.006

•An HPLC using microemulsion as the mobile phase has been developed.•The MELC were investigated detailed.•The first report MELC for the analysis of the four phenylethanoid glycosides in plasma sample. A simple, rapid and specific method was developed to separate as well as to determine the four phenylethanoid glycosides (PhGs) (echinacoside, tubuloside B, acteoside and isoacteoside) in rat plasma after oral administration of Cistanche salsa extract by reversed phase high performance liquid chromatography using a microemulsion as the mobile phase. The separations were performed on a Zorbax Extend-C18 column at 25°C. Photodiode-array detector was conducted at 322nm and with a flow rate of 0.8mLmin−1. The optimized microemulsion mobile phase consisted of 0.3% triethylamine in 20mM phosphoric acid at pH 6.0, 0.8% (v/v) ethyl acetate as oil phase, 1.5% (v/v) Genapol X-080 as surfactant, 2.5% (v/v) n-propanol as co-surfactant. Under the optimal conditions, the calibration curve for four PhGs was linear in the range of 10–1000ngmL−1 with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.64% and the limits of detection (LOD) for the four PhGs were 0.4–1.3ngmL−1 (S/N=3). The microemulsion liquid chromatography (MELC) method was successfully applied to separate and determine the four PhGs in rat plasma after oral administration of C. salsa extract.