Thermoelectric‐based temperature‐controlling system for in‐tube solid‐phase microextraction

A temperature‐controlling device for in‐tube solid‐phase microextraction was developed based on thermoelectric cooling and heating. This device can control the temperature of the capillary column from 0 to 100°C by applying a voltage to a Peltier cooler or stainless steel tube. The extraction temper...

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Bibliographic Details
Published in:Journal of separation science 2014-07, Vol.37 (13), p.1617-1621
Main Authors: Yang, Yang, Rodriguez‐Lafuente, Angel, Pawliszyn, Janusz
Format: Article
Language:English
Subjects:
Analysis
angiotensin I
Biological and medical sciences
Chromatography
Coolers
cooling
Desorption
detection limit
Devices
Drug analysis
Extraction
General pharmacology
heat
Heating
In-tube-solid phase microextraction
Linearity
Medical sciences
microextraction
Pharmacology. Drug treatments
propranolol
Ranitidine
solid phase microextraction
stainless steel
temperature
Thermoelectric control
Thermoelectric cooling
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Summary:A temperature‐controlling device for in‐tube solid‐phase microextraction was developed based on thermoelectric cooling and heating. This device can control the temperature of the capillary column from 0 to 100°C by applying a voltage to a Peltier cooler or stainless steel tube. The extraction temperatures for angiotensin I, propranolol, and ranitidine were optimized. In all cases, setting the temperature to 10°C for extraction achieved the best extraction efficiency. Desorption showed minimum peak broadening at 70°C, contributing to better chromatographic performance. Propranolol was selected as a model compound to compare the performance of temperature‐controlled in‐tube solid‐phase microextraction at optimized conditions. Calibration curves exhibited good linearity (R² > 0.999) over the studied range, and the limit of detection and limit of quantification were about three times lower than those obtained at standard conditions (30°C extraction and desorption).
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201400041