Maturation of mouse bone marrow dendritic cells (BMDCs) induced by Laminaria japonica polysaccharides (LJP)

• Published in: International journal of biological macromolecules 2014-08, Vol.69, p.388-392
• Main Authors: Meng, Jingjuan, Cao, Yan, Meng, Yiming, Luo, Hong, Gao, Xinghua, Shan, Fengping
• Format: Article
• Language: English
• Subjects: Acid Phosphatase - metabolism; Animals; Bone Marrow Cells - cytology; Bone marrow derived dendritic cells; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - metabolism; Immunologic Factors - pharmacology; Immunoregulation; Interleukin-12 - biosynthesis; Laminaria - chemistry; Laminaria japonica; Laminaria japonica polysaccharides; Maturation; Mice; Phagocytosis - drug effects; Polysaccharides - pharmacology; Tumor Necrosis Factor-alpha - biosynthesis; Up-Regulation - drug effects
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2014.05.018

•LJP could markedly induce structural, morphological and functional maturation of BMDCs.•The expression of key surface molecules on BMDCs was up-regulated and these molecules will help initiation of T cell immunity..•Induced BMDCs was accompanied with higher production of cytokine IL-12 and TNF-α, which will be involved in a chain of immune responses.•LJP is a potential immune stimulator to be used in cancer immunotherapy. The seaweed Laminaria japonica has been investigated in a laboratory research for its medical significance and LJP has been purified now. The objective of present study was to look at effect of LJP on structural, phenotypic and functional maturation of murine BMDCs. The structural maturation of BMDCs induced by LJP was evaluated by transmission electron microscopy (TEM); The phenotypic maturation of BMDCs was studied by flow cytometry(FCM) and functional maturation of BMDCs was analyzed by FITC-dextran, acid phosphatase (ACP) activity and enzyme linked immunosorbent assay (ELISA). We hereby proved that LJP markedly induced maturation of BMDCs with the data of decreased the number of lysosomes, upregulated expression of CD80, CD83, CD86, CD40 and MHC II key membrane molecules on BMDCs, downregulated phagocytosis, enriched production of IL-12 and TNF-α secreted by BMDCs. Therefore it should be concluded that LJP was with strong ability to induce maturation of BMDCs. Our data provided direct evidence to suggest that LJP could be considered as an immune stimulant in improving immune handicapped situation and as a useful adjuvant in vaccine designing.