Multilocus analysis using putative fungal effectors to describe a population of Fusarium oxysporum from sugar beet

Sugar beet (Beta vulgaris) Fusarium yellows is caused by Fusarium oxysporum f. sp. betae and can lead to significant reductions in root yield, sucrose percentage, juice purity, and storability. F. oxysporum f. sp. betae can be highly variable and many F. oxysporum strains isolated from symptomatic s...

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Bibliographic Details
Published in:Phytopathology 2014-08, Vol.104 (8), p.886-896
Main Authors: Covey, Paul A, Kuwitzky, Brett, Hanson, Mia, Webb, Kimberly M
Format: Article
Language:English
Subjects:
Alleles
Beta vulgaris
Beta vulgaris - microbiology
DNA Primers - genetics
DNA, Fungal - chemistry
DNA, Fungal - genetics
Fungal Proteins - genetics
Fusarium - genetics
Fusarium - isolation & purification
Fusarium - pathogenicity
Fusarium oxysporum
Genetic Markers - genetics
Genetic Variation
Genetics, Population
Genotype
Multilocus Sequence Typing
Mycological Typing Techniques
Phylogeny
Plant Diseases - microbiology
Plant Roots - microbiology
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Summary:Sugar beet (Beta vulgaris) Fusarium yellows is caused by Fusarium oxysporum f. sp. betae and can lead to significant reductions in root yield, sucrose percentage, juice purity, and storability. F. oxysporum f. sp. betae can be highly variable and many F. oxysporum strains isolated from symptomatic sugar beet are nonpathogenic. Identifying pathogenicity factors and their diversity in the F. oxysporum f. sp. betae population could further understanding of how this pathogen causes disease and potentially provide molecular markers to rapidly identify pathogenic isolates. This study used several previously described fungal effector genes (Fmk1, Fow1, Pda1, PelA, PelD, Pep1, Prt1, Rho1, Sge1, Six1, Six6, Snf1, and Ste12) as genetic markers, in a population of 26 pathogenic and nonpathogenic isolates of F. oxysporum originally isolated from symptomatic sugar beet. Of the genes investigated, six were present in all F. oxysporum isolates from sugar beet (Fmk1, Fow1, PelA, Rho1, Snf1, and Ste12), and seven were found to be dispersed within the population (Pda1, PelD, Pep1, Prt1, Sge1, Six1, and Six6). Of these, Fmk1, Fow1, PelA, Rho1, Sge1, Snf1, and Ste12 were significant in relating clade designations and PelD, and Prt1 were significant for correlating with pathogenicity in F. oxysporum f. sp. betae.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-09-13-0248-R