Development of an antibody-ELISA for seroprevalence of Trypanosoma evansi in equids of North and North-western regions of India

The importance of Trypanosoma evansi as the etiological agent for surra is often overlooked due to difficulty in accurate diagnosis of the disease. In the present study, an antibody-ELISA was developed using whole cell lysate antigen prepared from purified trypanosomes and used for seroprevalence st...

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Bibliographic Details
Published in:Veterinary parasitology 2013-09, Vol.196 (3-4), p.251-257
Main Authors: Kumar, Rajender, Kumar, Sanjay, Khurana, S.K., Yadav, S.C.
Format: Article
Language:English
Subjects:
Animals
antibodies
Antibodies, Protozoan - blood
Antibody-ELISA
antigens
blood
disease diagnosis
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Equidae
Equids
Equine
etiological agents
India - epidemiology
Seroepidemiologic Studies
Serologic Tests - methods
Serologic Tests - veterinary
Seroprevalence
Surra
Trypanosoma
Trypanosoma - classification
Trypanosoma - immunology
Trypanosoma evansi
Trypanosomiasis - epidemiology
Trypanosomiasis - parasitology
Trypanosomiasis - veterinary
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Summary:The importance of Trypanosoma evansi as the etiological agent for surra is often overlooked due to difficulty in accurate diagnosis of the disease. In the present study, an antibody-ELISA was developed using whole cell lysate antigen prepared from purified trypanosomes and used for seroprevalence study of T. evansi in equids. A total of 3695 equids were surveyed and blood samples were collected from each animal during September 2009 to August 2011. Out of these, 420 serum samples were found positive for presence of antibodies against T. evansi collected from equids of six agro-climatic zones of North and North-western regions of India comprising eight states viz., Gujarat (36/479), Haryana (11/275), Himachal Pradesh (14/83), Jammu and Kashmir (32/221), Punjab (1/38), Rajasthan (90/1148), Uttarakhand (141/753), and Uttar Pradesh (65/330). The maximum seroprevalence (19.69%) for T. evansi infection was observed in equids of Uttar Pradesh state with an overall seroprevalence of 11.36% in North and North-western regions of India. The results indicated that surra is endemic in equids of North and North-western parts of India.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2013.04.018