Three calpain isoforms are autolyzed in rat fast-twitch muscle after eccentric contractions

The present study investigated changes in autolysis of three calpain isoforms in skeletal muscles undergoing eccentric contractions (ECC), leading to prolonged force deficits. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC in situ, excised immediately af...

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Bibliographic Details
Published in:Journal of muscle research and cell motility 2014-04, Vol.35 (2), p.179-189
Main Authors: Kanzaki, Keita, Kuratani, Mai, Matsunaga, Satoshi, Yanaka, Noriyuki, Wada, Masanobu
Format: Article
Language:English
Subjects:
Animal Anatomy
Animals
Autolysis
Biomedical and Life Sciences
Biomedicine
Calpain - metabolism
Cell Biology
Histology
Life Sciences
Male
Morphology
Muscle Contraction - physiology
Muscle Fibers, Fast-Twitch - metabolism
Muscle Fibers, Fast-Twitch - physiology
Original Paper
Protein Isoforms
Proteomics
Rats
Rats, Wistar
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Summary:The present study investigated changes in autolysis of three calpain isoforms in skeletal muscles undergoing eccentric contractions (ECC), leading to prolonged force deficits. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC in situ, excised immediately after or 3 or 6 days after cessation of ECC, and used for measures of force output and for biochemical analyses. Full restoration of tetanic force in ECC-treated muscles was not attained until 6 days of recovery. Maximal calpain activity determined by a fluorogenic substrate was unaltered immediately after ECC, but increased to 313 and 450 % after 3 and 6 days, respectively. Increases in the amount of autolyzed calpain-3 were apparent immediately and developed progressively with recovery time, whereas elevations of autolyzed μ- and m-calpain occurred after 3 and 6 days, respectively. The protein content was augmented only in m-calpain. It is suggested that the three calpain isoforms may be involved in the dismantling, repair, remodeling and/or regeneration processes in ECC-treated muscles.
ISSN:0142-4319
1573-2657
DOI:10.1007/s10974-014-9378-9