Radiation degradation of (1→3)-β-d-glucan from yeast with a potential application as a plant growth promoter

• Published in: International journal of biological macromolecules 2014-08, Vol.69, p.165-170
• Main Authors: Luan, Le Quang, Uyen, Nguyen Huynh Phuong
• Format: Article
• Language: English
• Subjects: (1 → 3)-β-d-glucan; Gamma Rays; Glucans - chemistry; Glucans - isolation & purification; Glucans - pharmacology; Molecular Weight; Mustard Plant - drug effects; Mustard Plant - growth & development; Plant growth promotion; Plant Growth Regulators - chemistry; Plant Growth Regulators - isolation & purification; Plant Growth Regulators - pharmacology; Radiation degradation; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - cytology; Solubility; Water - chemistry
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2014.05.041

•In this study we prepared water-soluble oligo-(1→3)-β-D-glucan by γ-rays irradiation.•The properties of irradiated (1→3)-β-D-glucan were characterized by GPC, UV-visible spectroscopy, FTIR and NMR spectrophotometers.•The radiation-degraded (1→3)-β-D-glucan with Mw ∼ 18 kDa displayed a strongly promotion effect on the growth of mustard green.•The optimum concentration of the oligo-(1→3)-β-D-glucan was found to be about 75 mg l-1.•The oligo-(1→3)-β-D-glucan prepared by radiation technique displayed as a promising plant growth promoter for application in green agriculture. The (1→3)-β-d-glucan extracted from the yeast cell wall was irradiated by γ-rays from a Co-60 source at dose range of 100–300kGy in a swelling condition of 10, 15 and 20% for degradation. The water-soluble contents of irradiated samples obtained by 10% (1→3)-β-d-glucan mixture increased from 25.89 to 66.71% by the increasing of irradiation doses from 100 to 300kGy. While the molecular weight of the water-soluble (1→3)-β-d-glucan was found to be decreased from 48.13 to 10.77kDa. In the UV-spectra of irradiated water-soluble (1→3)-β-d-glucan, a new peak appeared at 265nm with the intensity increased by the increase of the dose. The IR spectra of irradiated (1→3)-β-d-glucan were recognized by a decrease of the peak intensity at 1156cm−1 indicated to COC glycosidic linkages with the increasing of irradiation dose. In addition, radiation degraded (1→3)-β-d-glucan with Mw about 18kDa prepared at the dose of 250kGy displayed a strongly promotion effect on the growth of mustard green and the optimum concentration of the degraded (1→3)-β-d-glucan was found to be about 75mgl−1. Thus, the degraded (1→3)-β-d-glucan prepared by radiation technique displayed as a promising, safety and high effective plant growth promoter for agriculture application.