Effects of passage number on proliferation and store-operated calcium entry in A7r5 vascular smooth muscle cells

Embryonic rat aortic smooth muscle cells, A7r5, have been used extensively as an in vitro vascular smooth muscle cell model. They are usually provided at 11th passage by supplier and generally used before 25th passage. However, the exact passage number (P#) used is not reported in general. In this s...

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Bibliographic Details
Published in:Journal of pharmacological and toxicological methods 2014-07, Vol.70 (1), p.1-5
Main Authors: Erac, Yasemin, Selli, Cigdem, Filik, Pelin, Tosun, Metiner
Format: Article
Language:English
Subjects:
Animals
Aorta, Thoracic - metabolism
Aorta, Thoracic - physiology
Calcium - metabolism
Cell culture
Cell growth
Cell Line
Cell Proliferation - physiology
Fura-2
Muscle, Smooth, Vascular - metabolism
Muscle, Smooth, Vascular - physiology
Myocytes, Smooth Muscle
Rats
RNA, Messenger - metabolism
Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism
Serotonin - metabolism
Subculturing method
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Summary:Embryonic rat aortic smooth muscle cells, A7r5, have been used extensively as an in vitro vascular smooth muscle cell model. They are usually provided at 11th passage by supplier and generally used before 25th passage. However, the exact passage number (P#) used is not reported in general. In this study, A7r5 cells (P#23) were used in quantitative gene (5-HT2A and SERCA2b) expression as well as in functional analyses including measurements of real-time changes in cell proliferation and in 5-HT- and CPA-induced intracellular Ca2+ levels. CPA-induced SR Ca2+ release and store-operated Ca2+ entry significantly increased (p23 cells (p23 cells (p
ISSN:1056-8719
1873-488X
DOI:10.1016/j.vascn.2014.03.001