Antidiabetic effect of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2014-09, Vol.30 (9), p.1061-1068
• Main Authors: Chen, Pei, Ph.D, Zhang, Qiuxiang, Ph.D, Dang, Hui, Ph.D, Liu, Xiaoming, Ph.D, Tian, Fengwei, Ph.D, Zhao, Jianxin, Ph.D, Chen, Yongquan, Ph.D, Zhang, Hao, M.A, Chen, Wei, Ph.D
• Format: Article
• Language: English
• Subjects: Animals; Antidiabetic effect; Antioxidants - metabolism; Biological and medical sciences; Blood glucose; Blood Glucose - metabolism; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - etiology; Diabetes. Impaired glucose tolerance; Diet, High-Fat; Dyslipidemias - drug therapy; Dyslipidemias - etiology; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Endotoxins - blood; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastroenterology and Hepatology; Glucose Intolerance - blood; Glucose Intolerance - drug therapy; Glucose Intolerance - etiology; Glycated Hemoglobin A - metabolism; Hypoglycemic Agents - therapeutic use; Insulin - blood; Islets of Langerhans - drug effects; Lactobacillus casei; Lactobacillus casei CCFM0412; Lipids - blood; Male; Malondialdehyde - blood; Medical sciences; Mice, Inbred C57BL; Oxidative Stress - drug effects; Pioglitazone; Streptozocin; Tumor Necrosis Factor-alpha - blood; Type 2 diabetes; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2014.03.022

Abstract Objective The aim of this study was to evaluate the antidiabetic effects of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin. Methods Thirty-two male C57 BL/6 J mice were assigned to four groups in this study. Type 2 diabetes was induced by feeding of a high-fat diet and injection of streptozotocin. L. casei CCFM0412 was administered to mice at a dose of 109  cfu/d per mouse for 12 wk. Body weight, fasting and postprandial 2-h blood glucose, oral glucose tolerance, glycosylated hemoglobin, insulin, and glycogen in liver were measured. Endotoxin, tumor necrosis factor-α, and interleukin-10 levels were determined. Lipid metabolic parameters including triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were also measured. The activities of glutathione peroxides, reactive oxygen species, and superoxide dismutase, and the levels of glutathione and malondialdehyde in the liver also were determined. Pancreas injury was evaluated by histologic analysis. Results At 13 wk, L. casei CCFM0412 significantly decreased fasting and postprandial 2-h blood glucose, glycosylated hemoglobin, endotoxin, tumor necrosis factor-α, triglycerides, total cholesterol, low-density lipoprotein cholesterol, reactive oxygen species, and malondialdehyde levels compared with the control group ( P