Polyhydroxyalkanoate biosynthesis and simultaneous remotion of organic inhibitors from sugarcane bagasse hydrolysate by Burkholderia sp

• Published in: Journal of industrial microbiology & biotechnology 2014-09, Vol.41 (9), p.1353-1363
• Main Authors: Lopes, Mateus Schreiner Garcez, Gomez, José Gregório Cabrera, Taciro, Marilda Keico, Mendonça, Thatiane Teixeira, Silva, Luiziana Ferreira
• Format: Article
• Language: English
• Subjects: Bacteria; Bagasse; Biochemistry; Bioenergy/Biofuels/Biochemicals; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Bioplastics; Biosynthesis; Biotechnology; Burkholderia; Burkholderia - growth & development; Burkholderia - isolation & purification; Burkholderia - metabolism; Carbon; Carbon sources; Cellulose - chemistry; Cellulose - metabolism; composite polymers; Ethanol; Experiments; Fundamental and applied biological sciences. Psychology; Genetic Engineering; hydrolysates; Industrial Microbiology; Inorganic Chemistry; levulinic acid; Life Sciences; Mechanical properties; Microbiology; Molecular Sequence Data; Organisms; Phylogeny; Poly-3-hydroxybutyrate; Polyhydroxyalkanoates; Polyhydroxyalkanoates - biosynthesis; R&D; Research & development; Saccharum - chemistry; Saccharum - metabolism; Saccharum - microbiology; soil; Soil Microbiology; Studies; Sugar; Sugarcane; sugarcane bagasse; xylose; Xylose - metabolism
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-014-1485-5

Burkholderia sp. F24, originally isolated from soil, was capable of growth on xylose and removed organic inhibitors present in a hemicellulosic hydrolysate and simultaneously produced poly-3-hydroxybutyrate (P3HB). Using non-detoxified hydrolysate, Burkholderia sp. F24 reached a cell dry weight (CDW) of 6.8 g L⁻¹, containing 48 % of P3HB and exhibited a volumetric productivity (PP₃HB) of 0.10 g L⁻¹ h⁻¹. Poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymers (P3HB-co-3HV) were produced using xylose and levulinic acid (LA) as carbon sources. In shake flask cultures, the 3HV content in the copolymer increased from 9 to 43 mol% by adding LA from 1.0 to 5.0 g L⁻¹. In high cell density cultivation using concentrated hemicellulosic hydrolysate F24 reached 25.04 g L⁻¹ of CDW containing 49 % of P3HB and PP₃HB of 0.28 g L⁻¹ h⁻¹. Based on these findings, second-generation ethanol and bioplastics from sugarcane bagasse is proposed.