Differential proteomics of dehydration and rehydration in bryophytes: evidence towards a common desiccation tolerance mechanism

• Published in: Plant, cell and environment cell and environment, 2014-07, Vol.37 (7), p.1499-1515
• Main Authors: CRUZ DE CARVALHO, RICARDO, BERNARDES DA SILVA, ANABELA, SOARES, RENATA, ALMEIDA, ANDRÉ M, COELHO, ANA VARELA, MARQUES DA SILVA, JORGE, BRANQUINHO, CRISTINA
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Amino Acid Sequence; aquatic habitat; Biological and medical sciences; Biomass; Bryopsida - metabolism; Cytoskeleton - metabolism; defense mechanisms; Dehydration; Desiccation; drought tolerance; Electrophoresis, Gel, Two-Dimensional; embryophytes; Energy Metabolism; Fontinalis; Fundamental and applied biological sciences. Psychology; Molecular Sequence Data; Peptides - chemistry; Peptides - metabolism; photosynthesis; Physcomitrella patens; Plant Proteins - chemistry; Plant Proteins - metabolism; Principal Component Analysis; Protein Biosynthesis; proteins; Proteome - chemistry; Proteome - metabolism; Proteomics; rehydration; Signal Transduction; Stress, Physiological; sucrose; Sucrose - metabolism; Tortula ruralis; Water - metabolism; water stress
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.12266

All bryophytes evolved desiccation tolerance (DT) mechanisms during the invasion of terrestrial habitats by early land plants. Are these DT mechanisms still present in bryophytes that colonize aquatic habitats? The aquatic bryophyte Fontinalis antipyretica Hedw. was subjected to two drying regimes and alterations in protein profiles and sucrose accumulation during dehydration and rehydration were investigated. Results show that during fast dehydration, there is very little variation in protein profiles, and upon rehydration proteins are leaked. On the other hand, slow dehydration induces changes in both dehydration and rehydration protein profiles, being similar to the protein profiles displayed by the terrestrial bryophytes Physcomitrella patens (Hedw.) Bruch and Schimp. and, to what is comparable with Syntrichia ruralis (Hedw.) F. Weber and D. Mohr. During dehydration there was a reduction in proteins associated with photosynthesis and the cytoskeleton, and an associated accumulation of proteins involved in sugar metabolism and plant defence mechanisms. Upon rehydration, protein accumulation patterns return to control values for both photosynthesis and cytoskeleton whereas proteins associated with sugar metabolism and defence proteins remain high. The current results suggest that bryophytes from different ecological adaptations may share common DT mechanisms.