Particle-associated extracellular enzyme activity and bacterial community composition across the Canadian Arctic Ocean

• Published in: FEMS microbiology ecology 2014-08, Vol.89 (2), p.360-375
• Main Authors: Kellogg, Colleen T.E., Deming, Jody W.
• Format: Article
• Language: English
• Subjects: Acetylglucosaminidase - chemistry; Aggregates; Arctic Ocean; Arctic Regions; Bacteria; Bacteria - classification; Bacteria - enzymology; Bacteria - genetics; Bacterial Proteins - chemistry; Canada; Carbon; Community composition; Ecology; Enzymatic activity; Euphotic zone; extracellular enzyme activity; Glucosidases - chemistry; Leucyl Aminopeptidase - chemistry; Microbial activity; Microbiology; Microbiota; Molecular Typing; Oceans and Seas; particle‐associated bacteria; Particulate organic carbon; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Seasons; Seawater - chemistry; Seawater - microbiology; Sequence Analysis, RNA; Water Microbiology
• ISSN: 0168-6496; 1574-6941
• DOI: 10.1111/1574-6941.12330

Abstract Microbial enzymatic hydrolysis of marine-derived particulate organic carbon (POC) can be a dominant mechanism for attenuating carbon flux in cold Arctic waters during spring and summer. Whether this mechanism depends on composition of associated microbial communities and extends into other seasons is not known. Bacterial community composition (BCC) and extracellular enzyme activity (EEA, for leucine aminopeptidases, glucosidases and chitobiases) were measured on small suspended particles and potentially sinking aggregates collected during fall from waters of the biologically productive North Water and river-impacted Beaufort Sea. Although other environmental variables appeared influential, both BCC and EEA varied along a marine productivity gradient in the two regions. Aggregates harbored the most distinctive bacterial communities, with a small number of taxa driving differences between particle-size classes (1.0–60 and > 60 μm) and free-living bacteria (0.2–1.0 μm). Significant relationships between patterns in particle-associated BCC and EEA suggest strong links between these two variables. Calculations indicated that up to 80% of POC in the euphotic zone of the North Water, and 20% in the Beaufort Sea, may be hydrolyzed enzymatically, underscoring the importance of this mechanism in attenuating carbon fluxes in Arctic waters even as winter approaches.