Evaluation of different approaches for modeling Escherichia coli O157:H7 survival on field lettuce

• Published in: International journal of food microbiology 2014-08, Vol.184, p.74-85
• Main Authors: McKellar, Robin C., Peréz-Rodríguez, Fernando, Harris, Linda J., Moyne, Anne-laure, Blais, Burton, Topp, Ed, Bezanson, Greg, Bach, Susan, Delaquis, Pascal
• Format: Article
• Language: English
• Subjects: biphasic model; Colony Count, Microbial; Computer Simulation; E. coli O157:H7; Escherichia coli; Escherichia coli O157 - physiology; field lettuce; Food Microbiology - methods; Lactuca - microbiology; Models, Statistical; predictive model; quantitative risk assessment
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2014.04.026

The ability to predict the behavior of Escherichia coli O157:H7 on contaminated field lettuce is essential for the development of accurate quantitative microbial risk assessments. The survival pattern of the species was assessed from several data sets derived from field-based experiments, which were analyzed by regression analysis fitting one monophasic model (log-linear) and two biphasic (Weibull and Cerf´s model) models. Probabilistic models were also simulated with @RISK™, integrating the fitted monophasic and biphasic models in order to analyze their impact on the estimate of the extent of die-off subsequent to a contamination event in the field. Regression analysis indicated that E. coli O157:H7 followed a biphasic decay pattern in most cases, with the Weibull and Cerf´s model showing similar good fit to individual and pooled survival data. Furthermore, results from the stochastic analysis demonstrated that using the log-linear model could lead to different risk estimates from those obtained with biphasic models, with a lower prevalence in the former scenario as no tailing is assumed in this model. The models and results derived from this work provide the first suitable mathematical base upon which to build probabilistic models to predict the fate of E. coli O157:H7 on field-grown leafy green vegetable. •E. coli O157:H7 survival on field lettuce followed a biphasic behavior.•Weibull and Cerf models were suitable to describe the E. coli O157:H7 survival.•Stochastic analysis indicated that the monophasic model led to lower risk estimates.