Loading…
Characterization of the extracellular biodemulsifiers secreted by Bacillus cereus LH-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions
A highly efficient demulsifying strain, LH-6, was isolated from petroleum-contaminated soil and identified as Bacillus cereus by 16S rDNA gene analysis. It achieved 95.61 and 95.40 % demulsifying ratios within 12 h for water-in-oil (W/O) and oil-in-water (O/W) model emulsions, respectively. Fourier...
Saved in:
| Published in: | Environmental science and pollution research international 2014-09, Vol.21 (17), p.10386-10398 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3 |
| container_end_page | 10398 |
| container_issue | 17 |
| container_start_page | 10386 |
| container_title | Environmental science and pollution research international |
| container_volume | 21 |
| creator | Hou, Ning Feng, Fengzhao Shi, Yan Cao, Huiming Li, Chunyan Cao, Zhi Cheng, Yi |
| description | A highly efficient demulsifying strain, LH-6, was isolated from petroleum-contaminated soil and identified as Bacillus cereus by 16S rDNA gene analysis. It achieved 95.61 and 95.40 % demulsifying ratios within 12 h for water-in-oil (W/O) and oil-in-water (O/W) model emulsions, respectively. Fourier transform infrared spectroscopy (FT-IR) and thin-layer chromatography (TLC) detections indicated that the LH-6’s extracellular biodemulsifiers were different types of lipopeptides for the W/O and O/W emulsions. Optimization of the culture medium composition was conducted to improve the biosynthesis and demulsifying efficiency of the biodemulsifier. The optimal carbon source was liquid paraffin, while waste frying oil could also be an alternative carbon source. The optimal nitrogen sources were ammonium sulfate and yeast extract. To further enhance the biodemulsifier efficiency, the optimal cultivation conditions were determined using response surface methodology (RSM) based on central composite rotation design (CCRD). Using the optimized cultivation conditions, the demulsifying ratios increased to 98.23 and 97.65 % for the W/O and O/W model emulsions, respectively. |
| doi_str_mv | 10.1007/s11356-014-2931-7 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1560139268</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1555249460</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3</originalsourceid><addsrcrecordid>eNqNkc-OFCEQxjtG466rD-BFO_HipZV_DcNRJ-qaTOJB90xoKGbYdMMI3RtnH8pnFNK7xngwHqCA-tUHxdc0zzF6gxESbzPGtOcdwqwjkuJOPGjOMS87waR82JwjyViHKWNnzZOcrxEiSBLxuDkjTAhBKTpvfm4POmkzQ_K3evYxtNG18wFa-DGXcxjHZdSpHXy0MC1j9s5Dym0Gk2AG2w6n9r02vmC5NZCghN1lx1sd7CoTDjoYmCDMVfle5OTDvgXnvPEQzKnKxOPsJ39bE7XQLOPsb9YnmRisr6v8tHnk9Jjh2V28aK4-fvi2vex2Xz593r7bdaZ0Pne9RM4iZp2gAy2zJgzQRvRS9mTDgRvnJCEbO8DQ96QfrGOyrB1wNBCiLb1oXq-6xxS_L5BnNflcf0MHiEtWuOcIU0n45j_QcgWTjKOCvvoLvY5LCqWRSjFGEaWiUHilTIo5J3DqmPyk00lhpKrvavVdFd9V9V3Vmhd3ysswgf1dcW90AcgK5JIKe0h_XP0P1ZdrkdNR6X3yWV19JQVAZfDSEP0FMsjFhw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1554430337</pqid></control><display><type>article</type><title>Characterization of the extracellular biodemulsifiers secreted by Bacillus cereus LH-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions</title><source>ABI/INFORM Collection</source><source>Springer Nature</source><creator>Hou, Ning ; Feng, Fengzhao ; Shi, Yan ; Cao, Huiming ; Li, Chunyan ; Cao, Zhi ; Cheng, Yi</creator><creatorcontrib>Hou, Ning ; Feng, Fengzhao ; Shi, Yan ; Cao, Huiming ; Li, Chunyan ; Cao, Zhi ; Cheng, Yi</creatorcontrib><description>A highly efficient demulsifying strain, LH-6, was isolated from petroleum-contaminated soil and identified as Bacillus cereus by 16S rDNA gene analysis. It achieved 95.61 and 95.40 % demulsifying ratios within 12 h for water-in-oil (W/O) and oil-in-water (O/W) model emulsions, respectively. Fourier transform infrared spectroscopy (FT-IR) and thin-layer chromatography (TLC) detections indicated that the LH-6’s extracellular biodemulsifiers were different types of lipopeptides for the W/O and O/W emulsions. Optimization of the culture medium composition was conducted to improve the biosynthesis and demulsifying efficiency of the biodemulsifier. The optimal carbon source was liquid paraffin, while waste frying oil could also be an alternative carbon source. The optimal nitrogen sources were ammonium sulfate and yeast extract. To further enhance the biodemulsifier efficiency, the optimal cultivation conditions were determined using response surface methodology (RSM) based on central composite rotation design (CCRD). Using the optimized cultivation conditions, the demulsifying ratios increased to 98.23 and 97.65 % for the W/O and O/W model emulsions, respectively.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-014-2931-7</identifier><identifier>PMID: 24777330</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Ammonium ; Ammonium sulfate ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Bacillus cereus ; Bacillus cereus - chemistry ; Bacillus cereus - metabolism ; Bacteria ; Biosynthesis ; Biotechnology ; Carbon ; Carbon sources ; Crude oil ; Cultivation ; culture media ; Earth and Environmental Science ; Ecotoxicology ; Efficiency ; Emulsions ; Emulsions - metabolism ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Extracellular Space - metabolism ; Fourier transform infrared spectroscopy ; Fourier transforms ; frying oil ; genes ; Infrared spectroscopy ; Kerosene ; Lipopeptides ; Lipopeptides - metabolism ; nitrogen ; Nitrogen sources ; Oil ; Oils ; Peptides ; Petroleum ; Research Article ; response surface methodology ; ribosomal DNA ; rRNA 16S ; soil ; Soil contamination ; Soil Microbiology ; Soil pollution ; Studies ; Thin layer chromatography ; Waste Water Technology ; Water ; Water Management ; Water Pollution Control ; Yeast ; yeast extract ; Yeasts</subject><ispartof>Environmental science and pollution research international, 2014-09, Vol.21 (17), p.10386-10398</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3</citedby><cites>FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1554430337/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1554430337?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11687,27923,27924,36059,36060,44362,74666</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24777330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hou, Ning</creatorcontrib><creatorcontrib>Feng, Fengzhao</creatorcontrib><creatorcontrib>Shi, Yan</creatorcontrib><creatorcontrib>Cao, Huiming</creatorcontrib><creatorcontrib>Li, Chunyan</creatorcontrib><creatorcontrib>Cao, Zhi</creatorcontrib><creatorcontrib>Cheng, Yi</creatorcontrib><title>Characterization of the extracellular biodemulsifiers secreted by Bacillus cereus LH-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>A highly efficient demulsifying strain, LH-6, was isolated from petroleum-contaminated soil and identified as Bacillus cereus by 16S rDNA gene analysis. It achieved 95.61 and 95.40 % demulsifying ratios within 12 h for water-in-oil (W/O) and oil-in-water (O/W) model emulsions, respectively. Fourier transform infrared spectroscopy (FT-IR) and thin-layer chromatography (TLC) detections indicated that the LH-6’s extracellular biodemulsifiers were different types of lipopeptides for the W/O and O/W emulsions. Optimization of the culture medium composition was conducted to improve the biosynthesis and demulsifying efficiency of the biodemulsifier. The optimal carbon source was liquid paraffin, while waste frying oil could also be an alternative carbon source. The optimal nitrogen sources were ammonium sulfate and yeast extract. To further enhance the biodemulsifier efficiency, the optimal cultivation conditions were determined using response surface methodology (RSM) based on central composite rotation design (CCRD). Using the optimized cultivation conditions, the demulsifying ratios increased to 98.23 and 97.65 % for the W/O and O/W model emulsions, respectively.</description><subject>Ammonium</subject><subject>Ammonium sulfate</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - chemistry</subject><subject>Bacillus cereus - metabolism</subject><subject>Bacteria</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Crude oil</subject><subject>Cultivation</subject><subject>culture media</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Efficiency</subject><subject>Emulsions</subject><subject>Emulsions - metabolism</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Extracellular Space - metabolism</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>frying oil</subject><subject>genes</subject><subject>Infrared spectroscopy</subject><subject>Kerosene</subject><subject>Lipopeptides</subject><subject>Lipopeptides - metabolism</subject><subject>nitrogen</subject><subject>Nitrogen sources</subject><subject>Oil</subject><subject>Oils</subject><subject>Peptides</subject><subject>Petroleum</subject><subject>Research Article</subject><subject>response surface methodology</subject><subject>ribosomal DNA</subject><subject>rRNA 16S</subject><subject>soil</subject><subject>Soil contamination</subject><subject>Soil Microbiology</subject><subject>Soil pollution</subject><subject>Studies</subject><subject>Thin layer chromatography</subject><subject>Waste Water Technology</subject><subject>Water</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>Yeast</subject><subject>yeast extract</subject><subject>Yeasts</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNqNkc-OFCEQxjtG466rD-BFO_HipZV_DcNRJ-qaTOJB90xoKGbYdMMI3RtnH8pnFNK7xngwHqCA-tUHxdc0zzF6gxESbzPGtOcdwqwjkuJOPGjOMS87waR82JwjyViHKWNnzZOcrxEiSBLxuDkjTAhBKTpvfm4POmkzQ_K3evYxtNG18wFa-DGXcxjHZdSpHXy0MC1j9s5Dym0Gk2AG2w6n9r02vmC5NZCghN1lx1sd7CoTDjoYmCDMVfle5OTDvgXnvPEQzKnKxOPsJ39bE7XQLOPsb9YnmRisr6v8tHnk9Jjh2V28aK4-fvi2vex2Xz593r7bdaZ0Pne9RM4iZp2gAy2zJgzQRvRS9mTDgRvnJCEbO8DQ96QfrGOyrB1wNBCiLb1oXq-6xxS_L5BnNflcf0MHiEtWuOcIU0n45j_QcgWTjKOCvvoLvY5LCqWRSjFGEaWiUHilTIo5J3DqmPyk00lhpKrvavVdFd9V9V3Vmhd3ysswgf1dcW90AcgK5JIKe0h_XP0P1ZdrkdNR6X3yWV19JQVAZfDSEP0FMsjFhw</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Hou, Ning</creator><creator>Feng, Fengzhao</creator><creator>Shi, Yan</creator><creator>Cao, Huiming</creator><creator>Li, Chunyan</creator><creator>Cao, Zhi</creator><creator>Cheng, Yi</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>20140901</creationdate><title>Characterization of the extracellular biodemulsifiers secreted by Bacillus cereus LH-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions</title><author>Hou, Ning ; Feng, Fengzhao ; Shi, Yan ; Cao, Huiming ; Li, Chunyan ; Cao, Zhi ; Cheng, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ammonium</topic><topic>Ammonium sulfate</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Bacillus cereus</topic><topic>Bacillus cereus - chemistry</topic><topic>Bacillus cereus - metabolism</topic><topic>Bacteria</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Carbon</topic><topic>Carbon sources</topic><topic>Crude oil</topic><topic>Cultivation</topic><topic>culture media</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Efficiency</topic><topic>Emulsions</topic><topic>Emulsions - metabolism</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Extracellular Space - metabolism</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Fourier transforms</topic><topic>frying oil</topic><topic>genes</topic><topic>Infrared spectroscopy</topic><topic>Kerosene</topic><topic>Lipopeptides</topic><topic>Lipopeptides - metabolism</topic><topic>nitrogen</topic><topic>Nitrogen sources</topic><topic>Oil</topic><topic>Oils</topic><topic>Peptides</topic><topic>Petroleum</topic><topic>Research Article</topic><topic>response surface methodology</topic><topic>ribosomal DNA</topic><topic>rRNA 16S</topic><topic>soil</topic><topic>Soil contamination</topic><topic>Soil Microbiology</topic><topic>Soil pollution</topic><topic>Studies</topic><topic>Thin layer chromatography</topic><topic>Waste Water Technology</topic><topic>Water</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>Yeast</topic><topic>yeast extract</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Ning</creatorcontrib><creatorcontrib>Feng, Fengzhao</creatorcontrib><creatorcontrib>Shi, Yan</creatorcontrib><creatorcontrib>Cao, Huiming</creatorcontrib><creatorcontrib>Li, Chunyan</creatorcontrib><creatorcontrib>Cao, Zhi</creatorcontrib><creatorcontrib>Cheng, Yi</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI商业信息数据库</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Proquest Health & Medical Complete</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Business Premium Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Ning</au><au>Feng, Fengzhao</au><au>Shi, Yan</au><au>Cao, Huiming</au><au>Li, Chunyan</au><au>Cao, Zhi</au><au>Cheng, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the extracellular biodemulsifiers secreted by Bacillus cereus LH-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>21</volume><issue>17</issue><spage>10386</spage><epage>10398</epage><pages>10386-10398</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>A highly efficient demulsifying strain, LH-6, was isolated from petroleum-contaminated soil and identified as Bacillus cereus by 16S rDNA gene analysis. It achieved 95.61 and 95.40 % demulsifying ratios within 12 h for water-in-oil (W/O) and oil-in-water (O/W) model emulsions, respectively. Fourier transform infrared spectroscopy (FT-IR) and thin-layer chromatography (TLC) detections indicated that the LH-6’s extracellular biodemulsifiers were different types of lipopeptides for the W/O and O/W emulsions. Optimization of the culture medium composition was conducted to improve the biosynthesis and demulsifying efficiency of the biodemulsifier. The optimal carbon source was liquid paraffin, while waste frying oil could also be an alternative carbon source. The optimal nitrogen sources were ammonium sulfate and yeast extract. To further enhance the biodemulsifier efficiency, the optimal cultivation conditions were determined using response surface methodology (RSM) based on central composite rotation design (CCRD). Using the optimized cultivation conditions, the demulsifying ratios increased to 98.23 and 97.65 % for the W/O and O/W model emulsions, respectively.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>24777330</pmid><doi>10.1007/s11356-014-2931-7</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-1344 |
| ispartof | Environmental science and pollution research international, 2014-09, Vol.21 (17), p.10386-10398 |
| issn | 0944-1344 1614-7499 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1560139268 |
| source | ABI/INFORM Collection; Springer Nature |
| subjects | Ammonium Ammonium sulfate Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Bacillus cereus Bacillus cereus - chemistry Bacillus cereus - metabolism Bacteria Biosynthesis Biotechnology Carbon Carbon sources Crude oil Cultivation culture media Earth and Environmental Science Ecotoxicology Efficiency Emulsions Emulsions - metabolism Environment Environmental Chemistry Environmental Health Environmental science Extracellular Space - metabolism Fourier transform infrared spectroscopy Fourier transforms frying oil genes Infrared spectroscopy Kerosene Lipopeptides Lipopeptides - metabolism nitrogen Nitrogen sources Oil Oils Peptides Petroleum Research Article response surface methodology ribosomal DNA rRNA 16S soil Soil contamination Soil Microbiology Soil pollution Studies Thin layer chromatography Waste Water Technology Water Water Management Water Pollution Control Yeast yeast extract Yeasts |
| title | Characterization of the extracellular biodemulsifiers secreted by Bacillus cereus LH-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T03%3A49%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20the%20extracellular%20biodemulsifiers%20secreted%20by%20Bacillus%20cereus%20LH-6%20and%20the%20enhancement%20of%20demulsifying%20efficiency%20by%20optimizing%20the%20cultivation%20conditions&rft.jtitle=Environmental%20science%20and%20pollution%20research%20international&rft.au=Hou,%20Ning&rft.date=2014-09-01&rft.volume=21&rft.issue=17&rft.spage=10386&rft.epage=10398&rft.pages=10386-10398&rft.issn=0944-1344&rft.eissn=1614-7499&rft_id=info:doi/10.1007/s11356-014-2931-7&rft_dat=%3Cproquest_cross%3E1555249460%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c499t-590fd04df73b3df7a24e0875995286e6cff9228dbeb5525bdf49bebfe60b22ad3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1554430337&rft_id=info:pmid/24777330&rfr_iscdi=true |