An outer membrane protein characteristic of mucoid strains of Pseudomonas aeruginosa

SDS‐polyacrylamide gel electrophoresis of outer membrane (OM) proteins of different mucoid strains of P. aeruginosa revealed a portion of about 54 kDa that was absent in nonmucoid strains. This 54 kDa protein was expressed under iron‐restricted and ironsufficient growth conditions. Electrophoretic m...

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Bibliographic Details
Published in:FEMS microbiology letters 1990-03, Vol.68 (1‐2), p.83-87
Main Authors: Grabert, Elmar, Wingeder, Jost, Winkler, Ulrich K.
Format: Article
Language:English
Subjects:
Alginate
Bacteriology
Biological and medical sciences
Cystic fibrosis
Fundamental and applied biological sciences. Psychology
gel electrophoresis
membrane proteins
Microbiology
Outer membrane protein
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pseudomonas aeuruginosa
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Summary:SDS‐polyacrylamide gel electrophoresis of outer membrane (OM) proteins of different mucoid strains of P. aeruginosa revealed a portion of about 54 kDa that was absent in nonmucoid strains. This 54 kDa protein was expressed under iron‐restricted and ironsufficient growth conditions. Electrophoretic mobility of the 54 kDa protein was modified by the solubilization temperature as well as by the addition of lipopolysaccharide and alginate prior to electrophoresis. Treatment of OMs with ocytlglucoside/KCl or SDS completely extracted the 54 kDa protein at low temperatures. The possible role of this protein in biosynthesis and/or excretion of bacterial alginate is discussed.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1990.tb04127.x