O super(6)-Ethylguanine carcinogenic lesions in DNA: An NMR study of O super(6)etG multiplied by C pairing in dodecanucleotide duplexes

The pairing of O super(6)etG with C located four base pairs in from either end of the self-complementary duplex (designated O super(6)etG multiplied by G 12-mer) has been investigated from an analysis of proton and phosphorus two-dimensional NMR experiments. The structural consequences of increasing...

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Bibliographic Details
Published in:Biochemistry (Easton) 1989-01, Vol.28 (15), p.6182-6192
Main Authors: Kalnik, M W, Li, BFL, Swann, P F, Patel, D J
Format: Article
Language:English
Online Access:Get full text
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Summary:The pairing of O super(6)etG with C located four base pairs in from either end of the self-complementary duplex (designated O super(6)etG multiplied by G 12-mer) has been investigated from an analysis of proton and phosphorus two-dimensional NMR experiments. The structural consequences of increasing the alkyl group size were elucidated from a comparative study of the pairing of O super(6)meG4 with C9 in a related (sequence designated O super(6)meG multiplied by C 12-mer). The NMR parameters for both O super(6)alkG-containing dodecanucleotides are also compared with those of the control sequence containing G4 multiplied by C9 base pairs (designated G multiplied by C 12-mer). The NOE cross-peaks detected in the two-dimensional NOESY spectra of the O super(6)alkG multiplied by C 12-mer duplexes in H sub(2)O solution establish that the O super(6)etG4/O super(6)meG4 and C9 bases at the lesion site stack into the helix between the flanking C3 multiplied by G10 and A5 multiplied by T8 Watson-Crick base pairs.
ISSN:0006-2960