Effect of transformation of Azotobacter vinelandii with the low copy number plasmid pRK290

We previously observed that when Azotobacter vinelandii was transformed by different broad-host-range plasmids, normal cellular functions such as growth and siderophore production are impaired. In the present work, when A. vinelandii was transformed with the low copy number plasmid pRK290, the exten...

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Bibliographic Details
Published in:Current microbiology 1989-09, Vol.19 (3), p.143-146
Main Authors: Glick, B.R. (University of Waterloo, Waterloo, Ontario, Canada), Bulter, B.J, Mayfield, C.I, Pasternak, J.J
Format: Article
Language:English
Subjects:
ADN
AZOTOBACTER
BACTERIA
BACTERIE
Bacteriology
Biological and medical sciences
DNA
Escherichia coli
FLORA DEL SUELO
FLORE DU SOL
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
PLASMIDE
PLASMIDIOS
PLASMIDS
SOIL FLORA
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Summary:We previously observed that when Azotobacter vinelandii was transformed by different broad-host-range plasmids, normal cellular functions such as growth and siderophore production are impaired. In the present work, when A. vinelandii was transformed with the low copy number plasmid pRK290, the extent of this metabolic impairment was lessened, as evidenced by increased siderophore production and moderate levels of growth on medium that lacks added iron. It is concluded that the severity of the plasmid-induced metabolic load reflects the relative level of expression of plasmid-encoded proteins.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01568932