Chronic Sleep Deprivation-Induced Proteome Changes in Astrocytes of the Rat Hypothalamus

Sleep deprivation (SD) can influence cognition, memory, and sleep/wake homeostasis and can cause impairments in many physiological processes. Because the homeostatic control of the sleep/wake cycle is closely associated with the hypothalamus, the current study was undertaken to examine proteomic cha...

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Bibliographic Details
Published in:Journal of proteome research 2014-09, Vol.13 (9), p.4047-4061
Main Authors: Kim, Jae-Hong, Kim, Jong-Heon, Cho, Young-Eun, Baek, Moon-Chang, Jung, Ji-Young, Lee, Maan-Gee, Jang, Il-Sung, Lee, Ho-Won, Suk, Kyoungho
Format: Article
Language:English
Subjects:
Animals
Astrocytes - chemistry
Astrocytes - metabolism
Hypothalamus - cytology
Hypothalamus - metabolism
Male
Proteome - analysis
Proteome - chemistry
Proteome - metabolism
Proteomics - methods
Rats
Rats, Sprague-Dawley
Sleep Deprivation - metabolism
Tandem Mass Spectrometry
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Summary:Sleep deprivation (SD) can influence cognition, memory, and sleep/wake homeostasis and can cause impairments in many physiological processes. Because the homeostatic control of the sleep/wake cycle is closely associated with the hypothalamus, the current study was undertaken to examine proteomic changes occurring in hypothalamic astrocytes following chronic partial SD. After chronic partial SD for 7 days, astrocytes were prepared from rat hypothalamus using a Percoll gradient method, and their proteome profiles were determined by LC–MS/MS. Comparisons of the proteome profiles of hypothalamic astrocytes revealed that chronic partial SD increased (≥1.5-fold) 89 proteins and decreased (≤0.7-fold) 50 proteins; these changes in protein expression were validated by western blot or immunohistochemistry. DAVID and IPA analyses of these proteins suggested that SD may influence gliotransmission and astrocyte activation. PPP2R1A, RTN4, VAMP-2, LGI-1, and SLC17A7 were identified and validated as the main targets of SD in astrocytes. Our results suggest that SD may modulate gliotransmission in the hypothalamus, thereby disturbing sleep/wake homeostasis and increasing susceptibility to neurological disease; however, further studies are required to confirm whether the proteome changes are specific to SD.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr500431j