Developmental patterns during direct somatic embryogenesis in protoplast cultures of european larch (Larix decidua Mill.)

Protoplasts isolated from embryogenic suspension cultures of European larch (Larix decidua Mill.) were cultured in thin alginate layers using a nylon mesh to enable a monitoring of the development of single cells. The patterns of cell division and differentiation are characterized and compared with...

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Bibliographic Details
Published in:Plant cell reports 1995-12, Vol.15 (3-4), p.242-247
Main Authors: KORLACH, J, ZOGLAUER, K
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Larix decidua
Methods. Procedures. Technologies
Plant cells and fungal cells
Protoplast preparation, cell fusion and regeneration
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Summary:Protoplasts isolated from embryogenic suspension cultures of European larch (Larix decidua Mill.) were cultured in thin alginate layers using a nylon mesh to enable a monitoring of the development of single cells. The patterns of cell division and differentiation are characterized and compared with zygotic embryogenesis to which homologies can only be drawn to some extent when the protoplasts grow in an auxinfree environment. Already at 2.5 μM both 2,4-dichlorophenoxyacetic acid or indole-3-acetic acid cause vacuolation and elongation of individual cells, thus disturbing the process of somatic embryogenesis which generally lacks the precise quantitative patterns occurring in vivo. Prior to the formation of an embryo, a proembryonal mass develops. Oligonucleated products of spontaneous protoplast fusions are able to cellularize even without preceding karyokinesis and perform a normal embryogenic program.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00193728