A chalcone synthase/stilbene synthase DNA probe for conifers

A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes...

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Bibliographic Details
Published in:Theoretical and applied genetics 1996-05, Vol.92 (7), p.827-831
Main Authors: BAKER, S. M, WHITE, E. E
Format: Article
Language:English
Subjects:
Abies procera
adn
Biological and medical sciences
calcona
chalcone
chalcones
Classical genetics, quantitative genetics, hybrids
dna
Fundamental and applied biological sciences. Psychology
gene
genes
Genetics of eukaryotes. Biological and molecular evolution
ligasas
ligase
ligases
Picea glauca
Picea sitchensis
Pinus lambertiana
Pinus monticola
Pseudotsuga menziesii
Pteridophyta, spermatophyta
Taxus brevifolia
Thuja plicata
Vegetals
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Summary:A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P. monticola, Picea glauca, P. sitchensis, Pseudostuga menziesii, Taxus brevifolia, and Thuja plicata. A large number of hybridizing bands were found in all species except T. plicata which did not cross hybridize. The hybridization patterns are highly polymorphic between the species and are also polymorphic within several of them.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00221894