Impact of bubble size on growth and CO2 uptake of Arthrospira (Spirulina) platensis KMMCC CY-007

• Published in: Bioresource technology 2014-10, Vol.170, p.310-315
• Main Authors: Kim, Kisok, Choi, Jaeho, Ji, Yosep, Park, Soyoung, Do, Hyungki, Hwang, Cherwon, Lee, Bongju, Holzapfel, Wilhelm
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Arthrospira (Spirulina) platensis; Bacterial Proteins - metabolism; Biological and medical sciences; Bubble size; Carbon - pharmacokinetics; Carbon Cycle - physiology; Carbon Dioxide - metabolism; CO2 uptake; Cyanobacteria; DNA Primers - genetics; Fluorescence; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - physiology; Multiprotein Complexes - metabolism; RNA, Messenger - metabolism; Spirulina - growth & development; Spirulina - metabolism
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.08.034

•CO2 bubble size influenced carbon uptake in Arthrospira platensis KMMCC CY-007.•mRNA expression levels varied according to uptake complexes and CO2 bubble size.•Low affinity NDH-I4 complex was strongly expressed under fine bubble.•NDH-I3 and also bicA and sbtA systems were weakly expressed under fine bubble. Optimisation of cyanobacterial cell productivity should consider the key factors light cycle and carbon source. We studied the influence of CO2 bubble size on carbon uptake and fixation, on basis of mRNA expression levels in Arthrospira platensis KMMCC CY-007 at 30°C (light intensity: 40μmolm−2s−1; 1% CO2). Growth rate, carbon fixation and lipid accumulation were examined over 7days under fine bubble (FB) (100μm Ø) bulk bubble (BB) (5000μm Ø) and non-CO2 (NB) aeration. The low affinity CO2 uptake mRNA (NDH-I4 complex) was stronger expressed than the high affinity NDH-I3 complex (bicA and sbtA) under 1% CO2 and FB conditions, with no expression of bicA1 and sbtA1 after 4days. The high affinity CO2 uptake mRNA levels corresponded to biomass, carbon content and lipid accumulation, and increase in NDH-I3 complex (9.72-fold), bicA (5.69-fold), and sbtA (10.61-fold), compared to NB, or BB conditions.