Reactivity of cloned, expressed human FcγRIII isoforms with monoclonal antibodies which distinguish cell-type-specific and allelic forms of FcγRIII

We have Isolated and expressed a cDNA encoding human NK cell FcγRIII. The NK cell cDNA differs from the neutrophil FcγRIII cDNA by a number of point mutations and encodes an additional 21 amino acids at Its C-terminus. When transiently expressed neutrophil and NK cell FcγRIII were digested with N-gl...

Full description

Saved in:
Bibliographic Details
Published in:International immunology 1990-04, Vol.2 (4), p.303-310
Main Authors: Trounstine, Mary L., Peltz, Gary A., Yssel, Hans, Huizinga, Tom W. J., von dem Borne, Albert E. G. Kr, Spits, Hergen, Moore, Kevin W.
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Antibodies, Monoclonal
Antigens, Differentiation - genetics
Cloning, Molecular
DNA - genetics
Epitopes - genetics
FcγRIII isoforms
genes
human
Humans
Immunoglobulin G
Killer Cells, Natural - immunology
leukocytes (neutrophilic)
mAbs
Molecular Sequence Data
Neutrophils - immunology
Polymorphism, Restriction Fragment Length
reactivity
Receptors, Fc - genetics
Receptors, IgG
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have Isolated and expressed a cDNA encoding human NK cell FcγRIII. The NK cell cDNA differs from the neutrophil FcγRIII cDNA by a number of point mutations and encodes an additional 21 amino acids at Its C-terminus. When transiently expressed neutrophil and NK cell FcγRIII were digested with N-glycanase, deglycosylated neutrophil FcγRIII had a more rapid electrophoretic mobility than NK cell FcγRIII, as is observed for the human FcγRIII isoforms on normal cells. The neutrophil and NK cell FcγRIII Isoforms apparently result from cell-type specific expression of different forms of FcγRIII mRNA. A Taql RFLP was also found for human FcγRIII. Monoclonal antibodies which have been used to distinguish the neutrophil and NK cell FcγRIII Isoforms and the NA1 and NA2 alleles of human neutrophil FcγRIII were employed to study the expression of two FcγRIII cDNA clones derived from neutrophlls and NK cells. FcγRIII encoded by the neutrophil-derlved cDNA reacts with the monoclonal antibody CLBgran11, while the NK-cell FcγRIII cDNA expresses the Fc receptor which carries an antigenic determinant recognized by the antibody GRM1. Characterization of hybrid FcγRIII constructed by interchange of restriction fragments between the neutrophil and NK cell cDNAs allowed localization of antigenic determiants recognized by the monoclonal antibodies.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/2.4.303