Human dermal fibroblast migration induced by fibronectin in autocrine and paracrine manners

Although fibronectin (FN) is known as a chemoattractant for human dermal fibroblasts (HDFs), it is unclear whether HDF migration is stimulated by FN produced by HDFs (autocrine manner) or by keratinocytes (paracrine manner). In this study, we investigated HDF migration by Boyden chamber assay using...

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Bibliographic Details
Published in:Experimental dermatology 2014-09, Vol.23 (9), p.682-684
Main Authors: Li, Xiaoguang, Qian, Hua, Ono, Fumitake, Tsuchisaka, Atsunari, Krol, Rafal P., Ohara, Koji, Hayakawa, Taihei, Matsueda, Satoko, Sasada, Tetsuro, Ohata, Chika, Furumura, Minao, Hamada, Takahiro, Hashimoto, Takashi
Format: Article
Language:English
Subjects:
Autocrine Communication
Cell Line
cell migration
Cell Movement - physiology
Culture Media, Conditioned
Fibroblasts - physiology
fibronectin
Fibronectins - physiology
human dermal fibroblasts
Humans
integrin
Integrin beta1 - physiology
Integrin beta3 - physiology
Keratinocytes - physiology
Paracrine Communication
Skin - cytology
Skin Physiological Phenomena
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Summary:Although fibronectin (FN) is known as a chemoattractant for human dermal fibroblasts (HDFs), it is unclear whether HDF migration is stimulated by FN produced by HDFs (autocrine manner) or by keratinocytes (paracrine manner). In this study, we investigated HDF migration by Boyden chamber assay using conditioned media from HDFs and HaCaT cells (keratinocyte cell line). Immunoblotting and enzyme‐linked immunosorbent assay revealed that FN existed in both conditioned media. Boyden chamber assay showed both conditioned media stimulated HDF migration, which was inhibited by anti‐FN antibody. Antibodies to both integrin β1and β3 subunits inhibited HDF migration induced by HDF‐conditioned medium almost completely and that by HaCaT cell‐conditioned medium with 50–60%. These results suggested that HDF migration was stimulated by FN in both autocrine and paracrine manners. However, the mechanisms of HDF migration by FN, particularly the role of integrin β1 and β3 subunits, were slightly different between autocrine and paracrine manners.
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.12447