Gamma irradiation of air-dried olive leaves: Effective decontamination and impact on the antioxidative properties and on phenolic compounds

• Published in: Food chemistry 2011-08, Vol.127 (3), p.1105-1113
• Main Authors: Aouidi, Fathia, Ayari, Samia, Ferhi, Hana, Roussos, Sevastianos, Hamdi, Moktar
• Format: Article
• Language: English
• Subjects: Antioxidant capacity; Biological and medical sciences; D10-value; Food engineering; Food industries; Fundamental and applied biological sciences. Psychology; Gamma irradiation; General aspects; Hygiene and safety; Olive leaf; Phenolic compound
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2011.01.109

► γ-irradiation at 20kGy is needed to decontaminate olive leaf. ► Microbial population in olive leaf is highly radioresistant (D10:9.74–25.12kGy). ► γ-irradiation maintains total phenolic, diphenols, flavonoids, oleuropein, verbascoside and rutin contents. ► γ-irradiation maintains the quality of phenols: TEAC, molecular mass distribution. ► Behavior of olive leaf after γ-irradiation isn’t affected by the form of the sample. Olive leaves are commercialized for their antioxidative value due to their valuable phenolic compounds. The present study aimed to evaluate the effect of gamma irradiation on microbial load, on antioxidative properties and on phenolic compounds of air-dried olive leaves. Irradiation was applied up to 25kGy (5kGy intervals) to powdered and intact samples. Total aerobic bacteria, yeast and mold, and lactic acid bacteria were counted after gamma irradiation. Decontamination was obtained at 20kGy. The radioresistance of microbial population was high with D10 values between 9.74 and 25.12kGy. Besides, gamma irradiation up to 25kGy was found to maintain the antioxidant capacity, molecular mass distribution of polyphenolics, total phenolics, ortho-diphenols, flavonoids, oleuropein, verbascoside and rutin contents. To conclude, the improvement of the microbial quality of air-dried olive leaves, without affecting phenolic composition and antioxidative properties, can be successively achieved by the application of gamma irradiation treatment.